摘要
以‘洛阳红’牡丹(Paeonia suffruticosa‘Luoyanghong’)切花为试材,研究10μL·L-1乙烯处理、1μL·L-1乙烯抑制剂1-MCP处理、90 g·L-1葡萄糖处理、90 g·L-1葡萄糖+10μL·L-1乙烯复合处理对切花花色和花青素苷含量的影响,以蒸馏水处理为对照。研究结果表明:乙烯处理的切花花色明度增加,红度和彩度下降,花瓣花青素苷含量下降;1-MCP处理与对照无显著差异;葡萄糖处理花色明度下降,红度和彩度增加,花瓣花青素苷含量增加;葡萄糖乙烯复合处理对切花花色及花青素苷积累也有明显改善与促进作用。对花青素苷合成相关基因表达量分析的结果表明,乙烯处理对基因表达有抑制作用,而葡萄糖处理有显著的正调控作用,葡萄糖乙烯复合处理则显著缓解了单独乙烯处理对基因表达的负调控作用。葡萄糖信号和乙烯信号之间一定程度上存在互作,葡萄糖缓解了乙烯对牡丹切花花青素苷合成的抑制作用。
The effects of different treatments（10 μL · L^-1 ethylene,1 μL · L^-1 ethylene inhibitor 1-MCP,90 g · L^-1 glucose and 90 g · L^-1 glucose in combination with 10 μL · L^-1 ethylene）on flower color and anthocyanin biosynthesis in the cut flowers of a tree peony（Paeonia suffruticosa）purplish red-flowered cultivar‘Luoyanghong＇were investigated. Results showed that flowers under ethylene treatment presented higher lightness,lower redness and chroma,as well as decreased anthocyanin content compared to ones from the control treatment. However,1-MCP treatment had no significant effects on the cut flowers. Glucose treatment prominently decreased lightness,increased redness and chroma of the flowers,and enhanced the anthocyanin accumulation. Similarly,glucose-ethylene combined treatment also marked beneficial effects on the coloration and anthocyanin accumulation. q RT-PCR was used to analyze the relative expression levels of anthocyanin biosynthetic genes and results showed that ethylene treatment significantly inhibited the expression of all tested genes,while glucose enhanced the expression of most genes. Glucose-ethylene combined treatment significantly up-regulated the genes,which would be inhibited by ethylene solo treatment. According to all above results,it can be concluded that petal coloration and anthocyanin biosynthesis in tree peony‘Luoyang Hong＇cut flower is negatively regulated by ethylene signaling but positively regulated by glucose signaling. Moreover,ethylene signaling and glucose signaling have an antagonism effect on anthocyanin biosynthesis,since glucose increased anthocyanin accumulation which was inhibited by ethylene.
出处
《园艺学报》
CAS
CSCD
北大核心
2015年第7期1356-1366,共11页
Acta Horticulturae Sinica
基金
高等学校博士学科点专项科研基金项目(20130014110014)
关键词
牡丹
切花
乙烯
葡萄糖
花色
花青素苷
Paeonia suffruticosa
cut flower
ethylene
glucose
flower color
anthocyanin
