摘要
为了揭示牡丹切花能量调控规律及其与切花瓶插品质的关系,以发育至二级状态的‘洛阳红’切花为试材,研究高能荷水平(大田自然生长,FNG)和低能荷水平(自然生长采后5~6℃贮藏15 d,PCS)状态下切花瓶插品质与能荷以及能量代谢的敏感基因SnRK1表达的关系。结果表明:(1)与FNG(高能荷)相比,PCS(低能荷)明显加快瓶插过程中牡丹切花开放和衰老进程,显著缩短瓶插寿命和减小最大花径,并提早呼吸跃变峰值,提高呼吸速率,加快蔗糖降解,导致花瓣琥珀酸脱氢酶(SDH)和线粒体复合体Ⅳ(CCO)活性下降加快,造成ATP和EC水平快速下降,引起PsSnRK1的表达水平上调时间提前,表达水平提高。(2)PsSnRK1基因能够对牡丹花瓣能量匮乏快速响应,能荷水平下降可诱导PsSnRK1基因表达上调。研究发现,冷藏后花瓣能荷水平下降是导致牡丹切花瓶插过程中花朵快速衰老的重要因素之一,花瓣能量状况在调控牡丹切花瓶插品质过程中起着重要作用,并为通过调节牡丹切花能量水平提高切花瓶插品质提供一种新策略。
In order to explore the energy regulation of cut tree peony flower and its relationship with the vase quality,the secondary stage‘Luoyanghong’cut tree peony flowers were applied as the experimental materials to investigate the relationship between the cut flower vase quality and the expression of SnRK1,a sensitive gene for energy charge and energy metabolism,under the high energy charge level(natural growth in the field,FNG)and low energy charge level(post-harvest of natural growth and stored at 5-6℃for 15 days,PCS).The results demonstrated that:(1)PCS accelerated the opening and senescence processes of cut tree peony flowers in the vase remarkably,and therefore,reduced the vase life and the maximum flower diameter significantly,comparing to that of FNG.In addition,PCS advanced the peak of respiratory climacteric and increased respiratory rate while accelerated sucrose degradation,which led to quick reduction of the activities of petal succinate dehydrogenase(SDH)and mitochondrial complex IV(CCO),rapid declines in ATP and EC levels,advanced PsSnRK1 gene up-regulation and increased expression level.(2)The decrease in energy charge level can induce the up-regulation of PsSnRK1 gene expression due to its quick respond to energy deficiency in tree peony petals.This study confirmed that the decrease in the energy charge level of petals after refrigeration was one of the important factors that led to the rapid senescence of the cut tree peony flower in vase process.The energy status of petals plays an important role in vase quality regulation of cut tree peony flower,which provides a new strategy to improve the vase quality of cut tree peony flower by regulating the cut flower's energy level.
作者
史田
孔秋真
秦绍玲
周爽
史国安
SHI Tian;KONG Qiuzhen;QIN Shaoling;ZHOU Shuang;SHI Guoan(Luoyang National Peony Park, Luoyang, He'nan 471003, China;Luoyang Key Labortary of Peony Biology, College of Mudan, Henan University of Science and Technology, Luoyang, He'nan 471023, China;3Greening Management Center of Luoyang, Luoyang, He'nan 471003, China)
出处
《西北植物学报》
CAS
CSCD
北大核心
2020年第9期1558-1565,共8页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家重点研发计划(2018YFD1000407)
国家自然科学基金(31372098)
河南省自然科学基金(162300410075)。
关键词
牡丹
切花
能荷状态
瓶插品质
tree peony
cut flower
energy status
vase quality
