摘要
以牡丹(Paeonia suffruticosa)品种'彩绘'为试材,采用RT-PCR和RACE方法从花瓣中获得了一个牡丹查耳酮合酶(chalcone synthase,CHS)基因cDNA全长,命名为Ps-CHS1,GenBank登录号为GQ483511。序列分析结果表明,Ps-CHS1全长1475bp,包含82bp的5′非编码区、208bp的3′非编码区和一个长度为1185bp编码394个氨基酸的开放阅读框。氨基酸序列分析显示该基因编码的蛋白具有CHS家族保守存在的所有功能活性位点和特征多肽序列。序列比对和系统进化分析表明,Ps-CHS1与杨柳科、锦葵科、蔷薇科等植物的CHS亲缘关系较近,相似性达90%以上。相对荧光定量PCR分析表明,Ps-CHS1在花瓣中的表达量最高,其次是萼片,再次是叶片和雄蕊,在心皮中表达量最低。
In this work, a full-length cDNA sequence of chalcone synthase （CHS） gene was obtained from petals of Paeonia suffruticosa ＇Caihui＇ using RT-PCR and RACE, named Ps-CHS1 （GenBank accession No. GQ483511） . Sequence analysis indicated that Ps-CHS1 is 1 475 bp in full length and contains a 5＇-untranslated region （5＇-UTR） of 82 bp, a 3＇-UTR of 208 bp, and an opening reading frame （ORF） of 1 185 bp encoding a 394 predicted amino acids residues which possessed all the conserved active sites for the CHS function as well as the family signature. Sequence alignment and phylogenetic analysis revealed that Ps-CHS1 shared more than 90% homology with CHS from plants in Salicaceae, Malvaceae and Rosaceae. Relative real-time PCR analysis indicated that Ps-CHS1 showed the highest transcript abundance in petals, moderate levels in sepals, low levels in leaves and stamens and the lowest levels in carpels.
出处
《园艺学报》
CAS
CSCD
北大核心
2010年第8期1295-1302,共8页
Acta Horticulturae Sinica
基金
国家‘863’项目(2006AA100109)
国家林业局‘948’项目(2006-4-C07)
关键词
牡丹
查耳酮合酶基因
相对荧光定量PCR
组织特异性表达
tree peony
Paeonia suffruticosa
chalcone synthase gene
relative real-time PCR
tissue-specific expression
