摘要
利用农杆菌介导的转基因技术,成功地将拟南芥抗冻转录激活因子基因CBF1转入粳稻中花11中,并获得了T1代转基因植株。CBF1基因及筛选基因HPT(潮霉素抗性基因)均在T1代中检测到,呈现单位点的孟德尔式遗传。常温和低温处理之后,T1代植株体内的脯氨酸含量均比野生型明显提高,同时,耐低温表型也在T1-1株系中出现。
In the present paper, using Agrobacterium-mediated transgene technique, an Arabidopsis anti-freeze transcriptional factor gene CBF1 was successfully introduced into japonic rice Zhonghua 11, and T1 genera-tion of transgenic rice was achieved. The CBF1 gene and the selection gene HPT were detected in the T1 seedlings,and these genes were in Mendelian single-copy inheredity. Both under normal and low temperature, the prolinecontents in transgenic rice were increased; meanwhile, some low-temperature-tolerance phenotype was observed.
出处
《细胞生物学杂志》
CSCD
2005年第1期73-76,共4页
Chinese Journal of Cell Biology
基金
国家自然科学基金(No.30400036)
浙江省自然科学基金(No.399505)资助项目~~
