摘要
以牡丹品种‘洛阳红’(Paeonia suffruticosa‘Luoyang Hong’)花瓣为材料,用CTAB法提取总RNA,根据已报道的ACC氧化酶(1-am inocyclopropane-1-carboxylic acid oxidase,ACO)保守氨基酸序列设计简并引物,通过RT-PCR扩增得到1条821bp的牡丹ACO基因同源片段。利用该已知中间序列,通过快速扩增cDNA末端技术(Race)及序列拼接,最终得到该基因cDNA全长序列,命名为Ps-ACO1,GenBank登录号为DQ337251。分析结果表明,Ps-ACO1 cDNA全长1221bp,包含一个939bp的开放读码框,5′非翻译区长65bp,3′非翻译区长217bp,编码产物为含有312个氨基酸残基的蛋白质。氨基酸序列与烟草、苹果、桃等植物的ACO同源性都达80%以上。
Total RNA was extracted from petals of ＇Luoyang Hong＇ tree peony（Paeonia suffruticosa）with CTAB method,and a pair of degenerated primer was designed based on the reported conserved amino acid sequence of 1-aminocyclopropane-1-carboxylic acid oxidase（ACO）.A cDNA fragment with 821 bp was amplified by RT-PCR,and then full length of it,named Ps-ACO1（GenBank No.DQ337251）,was obtained by RACE and sequence spliced.Sequence analysis indicated that Ps-ACO1 is 1 221 bp in full length and contains a 939 bp open reading frame flanking by a 65 bp 5＇-non-translation region and a 217 bp 3＇-non-translation region,and encodes a 312 predicated amino acid residues which shared more than 80% homology with ACO of tobacco,apple,peach and so on.
出处
《园艺学报》
CAS
CSCD
北大核心
2008年第6期891-894,共4页
Acta Horticulturae Sinica
基金
国家自然科学基金项目(30571309)
教育部新世纪优秀人才支持计划项目(NCET-05-0138)
