摘要
目的观察常氧和慢性间歇低氧下大鼠牙周炎模型中白细胞介素-18(IL-18)和低氧诱导因子(HIF)-1α的表达情况,探讨牙周炎和阻塞性睡眠呼吸暂停低通气综合征(OSAHS)发病相关的可能机制。方法将32只SD大鼠随机分为4组:常氧对照组、常氧牙周炎组、低氧对照组、低氧牙周炎组。通过结扎双侧上颌第二磨牙和高糖饮食的方法建立牙周炎模型。低氧对照组和低氧牙周炎组大鼠置于模拟中重度OSAHS的慢性间歇低氧环境中。8周后处死大鼠,检测各组大鼠的牙周指标,采用酶联免疫吸附测定法检测各组大鼠血清及牙龈组织中IL-18和HIF-1α的质量浓度,分析低氧牙周炎组的牙周附着丧失与IL-18和HIF-1α的相关性。结果低氧牙周炎组的IL-18、HIF-1α的质量浓度较其余3组明显升高(P<0.05);其血清中IL-18、HIF-1α的质量浓度与牙周附着丧失呈正相关,相关系数r分别为0.792和0.753(P<0.05);牙龈组织中IL-18、HIF-1α质量浓度与牙周附着丧失也呈正相关,相关系数r分别为0.817和0.779(P<0.05)。结论慢性间歇低氧能加重牙周炎症,与IL-18、HIF-1α的表达有相关性。
Objective This study evaluates the expression of interleukin-18（IL-18） and hypoxia-inducible factor（HIF）-1α in rat periodontitis model exposed to normoxia and chronic intermittent hypoxia（CIH） environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome（OSAHS） was also investigated. Methods Thirty-two Sprague–Dawley（SD） rats were randomly assigned into four groups： normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1α in serum and gingival tissues were determined using enzyme-linked immunosorbent assay（ELISA）. The correlation between attachment loss（AL） and the levels of IL-18 and HIF-1α in hypoxia periodontitis group was evaluated. Results The levels of IL-18 and HIF-1α in hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups（P0.05）. Furthermore, the levels of IL-18 and HIF-1α in serum（r=0.792, r=0.753, P0.05） and gingival tissues（r=0.817, r=0.779, P0.05） were positively correlated with AL. Conclusion CIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-1α levels.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2015年第4期383-387,共5页
West China Journal of Stomatology
基金
山西省自然科学基金资助项目(2014011046-2)
