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经眶下孔注射相关炎症因子制造大鼠三叉神经痛模型的实验研究 被引量:10

An experimental study of injecting inflammatory cytokines through infraorbitalfossa to manufacture rat model of trigeminal neuralgia
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摘要 目的经SD大鼠眶下孔注射炎症因子肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)以制造一种新型的大鼠三叉神经痛(TGN)模型。方法将大鼠随机分为4组,即生理盐水组(NS组)、TNF-α组、IL-1β组、TNF-α+IL-1β组;各组分别于大鼠眶下孔注射等量的生理盐水和TNF-α或IL-1β。观察并比较注射后不同时段各组大鼠注射侧触须垫机械痛阈、自发行为学改变以及电镜观察组织改变。结果从注射后3 d至注射后8周,各炎症因子注射组大鼠均出现注射侧触须垫痛觉过敏及搔抓面部次数增多,同NS组相比,差异有统计学意义(P<0.05);各炎症因子注射组间差异无统计学意义。各炎症因子注射组大鼠眶下神经及三叉神经节均出现不同程度的脱髓鞘改变,而NS组大鼠则无脱髓鞘现象。结论利用炎症因子TNF-α、IL-1β经大鼠眶下孔注射,可使大鼠产生类似TGN症状和病理学变化,该TGN模型更接近于临床且造模方法简便。 Objective To establish a rat model of trigeminal neuralgia by injecting inflammatory cytokines,TNF-αand IL-1β through infraorbital foramen. Methods Rats were randomly divided into four groups:normal saline group,TNF-α group,IL-1β group and TNF-α + IL-1β group. Rats in each group were injected with the same e-quivalent of normal saline and TNF-α or IL-1β,respectively. The mechanical withdrawal threshold of the injecting side of vibrissa pad,video detection of spontaneous behavior and the organizational change in electron microscopy were observed and compared in each group of rats. Results From 3 d to 8 w after injection,rats in all cytokines injecting groups appeared hyperalgesia in the vibrissa pad. And there was an increased frequency of scratching the face at the same time. Compared with the normal saline group,it was significantly different(P 〈 0. 05). However, there was not significant difference among TNF-α group,IL-1β group and TNF-α + IL-1β group. Nerve demyeli-nations were observed in both infraorbital nerve and trigeminal ganglion in all cytokines injecting group,while there was no demyelination in the normal saline group. Conclusion Injecting cytokines,TNF-α and / or IL-1β can make a trigeminal neuralgia model which is easy to operate and very close to the trigeminal neuralgia in clinic.
出处 《安徽医科大学学报》 CAS 北大核心 2015年第6期753-756,共4页 Acta Universitatis Medicinalis Anhui
基金 国家自然科学基金(编号:81271162) 安徽省自然科学基金(编号:11040606M204) 高等学校省级优秀青年人才基金(编号:2012SQRL070)
关键词 三叉神经痛 动物模型 炎症因子 脱髓鞘 眶下孔注射 trigeminal neuralgia animal model inflammatory cytokines demyelination infraorbital foramen in-jection
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参考文献13

  • 1崔悦,赵佳,王晔,孙丹丹,张莹,刘洋,赵小亮,牛晓红,张美玉,王丹巧,高天乐,徐晓军.一种光化学损伤诱导的三叉神经痛大鼠模型的建立[J].中国药理学通报,2014,30(7):1026-1030. 被引量:8
  • 2孙晖,李国超(综述),王元银,王烈成(审校).眶下神经慢性缩窄环三叉神经痛动物模型的研究进展[J].医学综述,2013,19(12):2113-2116. 被引量:4
  • 3张亮..局部施用辣椒素治疗原发性三叉神经痛的实验研究[D].东南大学,2007:
  • 4Christensen D, Gautron M, Guilbaud G, et al. Combined system- ic administration of the glycine/NMDA receptor antagonist, ( + )- HA966 and morphine attenuates pain-related behaviour in a rat model of trigeminal neuropathic pain [ J]. Pain, 1999, 83 ( 3 ) : 433 - 40. 被引量:1
  • 5Vos B P, Strassman A M, Maciewicz R J. Behavioral evidence of trigeminal neuropathie pain following chronic constriction injury to the rat' s infraorbital nerve [ J]. J Neurosci, 1994,14 ( 5 Pt 1 ) : 2708 - 23. 被引量:1
  • 6Falchi M, Ferrara F, Gharib C, et al. Hyperalgesic effect of in- trathecally administered interleukin-1 in rats [ J ]. Drugs Exp Clin Ptes ,2001,27 (3) :97 - 101. 被引量:1
  • 7Reeve A J, Patel S, Fox A, et al. Intrathecally administered en- dotoxin or cytokines produce allodynia, hyperalgesia and changesin spinal cord neuronal responses to nociceptive stimuli in the rat [J]. Eur J Pain,2000,4(3) :247 -57. 被引量:1
  • 8毛吉刚,陈根元,萧文耀,陈睿,陆军.大鼠腰神经根性疼痛相关行为变化与硬膜外注射白细胞介素1受体拮抗剂干预的影响[J].中国临床康复,2005,9(18):129-131. 被引量:3
  • 9Wagner R, Myers R R. Endoneurial injection of TNF-alpha pro- duces neuropathic pain behaviors [ J]. Neuroreport, 1996, 7 (18) : 2897 -901. 被引量:1
  • 10Shi X, Chen Y, Nadeem L, et al. Beneficial effect of TNF-c inhi- bition on diabetic peripheral neuropathy [ J ]. J Neuroinflamma- tion, 2013, 10: 69. 被引量:1

二级参考文献27

  • 1王福,张奎启,裘罡.三叉神经痛的病因和发病机制学研究的进展[J].大连医科大学学报,2004,26(4):307-310. 被引量:7
  • 2周瑸,刘玉光,吴承远.建立大鼠三叉神经痛动物模型[J].山东医药,2005,45(3):21-22. 被引量:5
  • 3王惠岚,罗道枢,陈江.大鼠三叉神经痛模型的建立[J].福建医科大学学报,2006,40(5):520-522. 被引量:5
  • 4黄云巧,杨建明,陈国强.谷氨酸与慢性疼痛[J].昆明医学院学报,2007,28(02B):117-121. 被引量:5
  • 5Wehling P, Cleveland SJ, Heininger K, et al. Neurophysiologic changes in lumbar nerve root inflammation in the rat after treatment with cytokine inhibitors. Spine 1996;21(8):931 -5 被引量:1
  • 6Tamasaski Y, Matsuura N, Shozuhara H, et al. Interleukin-1 as a pathogenetic mediator of ischemic brain damage in rats. Stroke 1995; 26(9): 676-81 被引量:1
  • 7Hayashi N, Weinstein J, Meller ST, et al. The effect of epidural injection of betamethasone or bupivacaine in a rat model of lumbar radiculopathy. Spine 1998;23(8):877 -85 被引量:1
  • 8Saal JS. The role of inflammation in lumbar pain. Spine 1995 ;20(16): 1821 -7 被引量:1
  • 9Arend WP. The balance between IL-1 and IL-1Ra in disease. Cytokine Growth Factor 2002; 13 (4-5 ): 323-40 被引量:1
  • 10Kawakami M, Matsumoto T, Hashizume H, et al. Epidural injection of cyclooxygenase-2 inhibitor attenuates pain-related behavior following application of nucleus pulposus to the nerve root in the rat. J Orthopaedic Research 2002; 20(2):376-81 被引量:1

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