摘要
目的观察光化学损伤诱导的三叉神经痛(PITN)大鼠模型的行为学、周围和中枢神经系统疼痛相关递质/调质的变化及对药物的反应,为TN的基础研究提供可用的动物模型。方法将适应性刺激筛选合格的SD♂大鼠随机分为假手术对照组、TN模型组、TN加巴喷丁组(100 mg·kg-1),模型以赤藓红B静脉注射加激光照射方法诱发。采用Von Frey hairs测量大鼠机械痛阈值,评价其行为学变化;荧光定量PCR技术检测三叉神经节内Tac1 mRNA表达量,脑内微透析方法采集脑纹状体细胞外液,高效液相-荧光法检测其中谷氨酸(Glu)的水平。结果大鼠三叉神经支配的颜面部位机械痛阈平均值于造模d 7后稳定在4 g以下,模型成功率为63%;造模前后自身对照,痛阈值由26 g降低为(1.60±1.74)g(P<0.01);术后d 10模型组(1.63±1.27 g)与假手术对照组(24.17±4.49 g)比较明显下降(P<0.01);造模后d 7-60内动态观察显示其均值稳定在(0.71±1.24)g。模型组与假手术对照组比较三叉神经节内Tac1 mRNA表达量明显上调,纹状体细胞外液Glu水平明显升高(均P<0.05)。加巴喷丁使其疼痛行为学指标明显改善、Tac1 mRNA表达量下调、Glu水平降低(均P<0.05)。结论光化学诱导的TN大鼠模型可以模拟TN的临床症状及部分病理生理机制,模型的稳定性良好,可用于TN的实验研究及治疗药物的筛选。
Aim To investigate the behavioral changes of the pain related neuromodulation and neurotransmission in peripheral and central nervous systems in rats with trigeminal neuralgia （TN）and provide a disease relevant animal model for mecha-nism study of TN.Methods The male SD rats were randomly divided into sham operation group and TN surgical group.The latter group was further divided into model group and gabapentin group （100 mg ＆#183; kg-1 ）. TN was induced by intravenous erythrosine B injection and laser irradiation.The pain behavior of rats was evaluated using mechanical pain threshold measured with Von Frey hairs.Fluorescence quantitative PCR technique was deployed to study the change of Tac1 mRNA expressions in trigeminal ganglia.Utilizing microdialysis technique followed by high performance liquid chromatography fluorescence detection （HPLC-FLD）,the extracellular striatum fluid was collected and glutamate（Glu）concentration was determined.Results In the model group,the average mechanical pain threshold in facial ar-ea innervated by the trigeminal nerve remained below 4g after 7 days post surgery.The mechanical threshold of the model group （1.63 ±1.27）g was significantly lower （P〈0.01）than the“nbsp;control group （24.17 ±4.49）g on day10 post surgery.In gen-eral,the mechanical withdraw threshold was decreased from the preoperative value of 26g to the postoperative value of （1.60 ± 1.74）g （P〈0.01）,and maintained stable at （0.71 ±1.24） g during the whole dynamic monitoring period from day7 to day60.The successful rate of this model was 63%.After sur-gery,Tac1 mRNA expression in trigeminal ganglia and extracel-lular Glu levels in striatum were significantly up-regulated （P〈0.05 ） in the model group. Animals receiving Gabapentin showed significant improvement in pain symptoms,as well as re-ductions of Tac1 mRNA expression in trigeminal ganglia and ex-tracellular Glu concentration in striatum （P〈0.05 ）.Conclu-sions The above described photochemically induce
出处
《中国药理学通报》
CAS
CSCD
北大核心
2014年第7期1026-1030,共5页
Chinese Pharmacological Bulletin
基金
国家科技部国际科技合作项目(No 2010DFA31890)
中国中医科学院自主选题研究(No ZZ2013003)
