摘要
目的:探讨长期慢性高原暴露时不同海拔牦牛(Yak)及黄牛(Cattle)血液主要肝酶指标变化及其与ACE/ACE2比值的相关性。方法:采集青海不同地区的牦牛血样,按海拔高度分为3 000 m、3 500 m、4 000 m及4 300 m等4个组,同时采集高山迁饲黄牛(2 500 m)及低海拔黄牛(1 300 m)血液,利用全自动血液生化分析仪测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、胆碱酯酶(CHE)、谷氨酰胺转移酶(GGT)、碱性磷酸酶(ALP)、血清脂肪酶(LPS)水平,并测定血清血管紧张素转化酶(ACE)、ACE2水平,利用单因素方差分析法分析不同海拔高度的牦牛之间,及高山迁饲黄牛和低海拔黄牛之间上述肝酶指标的差异性,并对三种牛血清中肝酶指标与ACE/ACE2比值的相关性进行分析。结果:与低海拔相比,4 000m组及4 300 m组牦牛血清ALT单项升高较显著,而AST、CHE、GGT、ACE/ACE2比值等指标在不同海拔牦牛血清中无明显变化。与低海拔黄牛相比,高山迁饲黄牛血清中AST、CHE活性显著升高,LPS、ACE活性显著降低,尤其是ACE/ACE2比值降低近2倍。相关性分析表明,牦牛血清LPS水平与ACE/ACE2比值显著相关(r=0.357,P<0.01),低海拔黄牛ALP水平与ACE/ACE2比值显著相关(r=0.418,P<0.05),但ACE/ACE2比值的改变对肝酶指标改变的最大贡献率仅为17.5%。结论:长期慢性低氧时高山土生牦牛血液肝酶活性受海拔高度影响不明显,黄牛血清肝酶活性随海拔变化较明显,这些变化与ACE/ACE2比值变化无实际相关性。
Objective： To investigate the difference of liver enzyme levels and its correlation with serum ACE/ACE2 among yak and cattle on Qinghai-Tibetan plateau, and to further explore the biochemical mechanism of their liver of altitude adaptation. Methods： The serum sam- pies of yak were collected at 3 000 m, 3 500 m, 4 000 m and 4 300 m respectively, meanwhile the serum samples of migrated cattle on plateau （2 500 m） and lowland cattle （1 300 m） were also collected. The levels of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, cholinesterase （CHE）, gamma glutamyl transferase（GGT）, alkaline phosphatase （ALP）, serum lipase （LPS）, angiotensin converting enzyme（ACE）, angiotensin converting enzyme-2 （ACF2）in serum were measured by using fully automatic blood biochemical analyzer. We analysed the differences of the above enzymes and its correlation with ACE/ACFe. We used one way analysis of variance （A_NOVA）. Results： The levels of ALT in 4 000 m group and 4 300 m group of yak increased significantly compared with other groups, there were no statistically significant differences in AST, CHE, GGT, ACE/ACF~ levels of yaks at different altitudes. As compared to lowland cattle, the serum levels of AST and CHE were increased, the level of LPS and ACE was decreased significantly, respectively, and especially, the ratio of ACE/ACE2 of migranted cattle reduced nearly two times. The levels of LPS were significantly correlated to the ratio of ACE/ACF2 in yak（ r = 0.357, P 〈 0.01）, and a high correlation between ALP and ACE/ACE2 in lowland cattle（ r = 0.418, P 〈 0.05）, But the biggest contribution rate of the ratio of ACE/ACEe was only 17.5% for the changes of the levels of liver enzyme. Conclusion： The results indicated that with the altitude in- creased did not significantly influence the changes of liver enzymes＇ activities in mountainous yaks but not in cattle. However, all above these changes weren＇t actually correlated to the ratio of ACE/ACE2.
出处
《中国应用生理学杂志》
CAS
CSCD
2015年第3期272-275,共4页
Chinese Journal of Applied Physiology
基金
科技部973前期资助项目(2012CB722506)
科技部973资助项目(2012CB518202)
