摘要
目的检测肺癌患者外周血调节性B细胞(CD19+CD5+CD1d+B细胞,Bregs)的表达及其临床意义。方法收集72例肺癌患者和29例健康体检者的外周血,采用流式细胞仪检测Bregs细胞数量,ELISA法检测外周血清IL-10和TGF-β的水平。比较肺癌患者与健康对照,肺癌Ⅰ+Ⅱ期与Ⅲ+Ⅳ期患者Bregs细胞比例、外周血IL-10和TGF-β水平的差异;分析肺癌患者Bregs细胞比例与外周血IL-10和TGF-β水平的相关性。结果肺癌患者外周血Bregs细胞比例明显高于健康对照(5.01%±1.20%vs 2.78%±0.56%,P<0.01),Ⅲ+Ⅳ期患者高于Ⅰ+Ⅱ期患者(5.63%±1.04%vs 4.13%±0.78%,P<0.01)。肺癌患者外周血清IL-10和TGF-β水平明显高于健康对照(2.34±0.79pg/ml vs 1.29±0.51pg/ml,56.64±6.93ng/ml vs 22.42±4.42ng/ml,P<0.01),Ⅲ+Ⅳ期患者高于Ⅰ+Ⅱ期患者(2.79±0.60pg/ml vs 1.71±0.59pg/ml,59.88±6.63ng/ml vs 52.10±4.37ng/ml,P<0.01)。相关分析显示,肺癌患者外周血Bregs细胞比例与IL-10水平呈正相关(r=0.69,P<0.01),而与TGF-β水平无明显相关性。结论肺癌患者外周血Bregs细胞比例及血清IL-10、TGF-β水平增高,提示Bregs细胞可能与肺癌的进展相关。
Objective To investigate the expression and clinical significance of regulatory B cells（Bregs） in peripheral blood of patients with lung cancer. Methods The peripheral blood was collected from 72 patients with lung cancer and 29 healthy subjects（as control）, and the Bregs subsets（CD19＋CD5＋CD1d＋） were detected by flow cy tometr y, the levels of IL-10 and TGF-β in serum were determined by ELISA. Proportion of Bregs and peripheral blood levels of IL-10 and TGF-β were compared between lung cancer patients and healthy controls, and between the lung cancer patients in stage Ⅰ＋Ⅱ and those in stage Ⅲ＋Ⅳ. The correlation between Bregs proportion and peripheral blood levels of IL-10 and TGF-β were further investigated. Results The percentage of Bregs was significantly higher in the peripheral blood of lung cancer patients than that of healthy controls（5.01%±1.20% vs 2.78%±0.56%, P〈0.01）. The proportion of Bregs was found to be increased in patients in clinical Ⅲand Ⅳ stages（5.63%±1.04%） compared with those in clinical Ⅰ and Ⅱ stages（4.13%±0.78%, P〈0.01）. The IL-10 and TGF-βlevels were obviously higher in lung cancer patients than those in healthy controls（2.34±0.79pg/ml vs 1.29±0.51pg/ml, and 56.64±6.93ng/ml vs 22.42±4.42ng/ml, respectively, P〈0.01）, and in patients in Ⅲ and Ⅳ stages than those in patients in Ⅰ and Ⅱ stages（2.79±0.60pg/ml vs 1.71±0.59pg/ml, and 59.88±6.63ng/ml vs 52.10±4.37ng/ml, respectively, P〈0.01）. There was a positive correlation between the proportion of Bregs and the levels of IL-10（r=0.69, P〈0.01）, but no significant correlation was found between the proportion of Bregs and the levels of TGF-β. Conclusion Increased proportion of Bregs and levels of serum IL-10 and TGF-β in lung cancer patients may imply that the Bregs are probably associated with the progression of lung cancer.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2015年第5期396-399,共4页
Medical Journal of Chinese People's Liberation Army
基金
浙江省新世纪151人才工程基金(台人社发[2011]121号)~~
