摘要
目的:检测宫颈癌患者外周血调节性B细胞的数量以及血清中IL-10及TGF-β的表达水平,探讨Br1在宫颈癌临床监测中的意义。方法:收集50例宫颈癌患者外周血标本,用间接免疫荧光法检测Br1细胞亚群的分布,用酶联免疫吸附试验(ELISA)检测血清中IL-10及TGF-β的表达情况。结果:宫颈癌患者外周血Br1的数量明显高于正常对照组,IL-10的含量均明显高于正常对照组(P<0.05),宫颈癌浸润组与正常对照组相比,Br1的数量及IL-10明显升高(P<0.05)。结论:宫颈癌患者中Br1的数量及血浆IL-10明显升高,提示Br1细胞与宫颈癌的发生发展可能存在着一定的关系。
Objective：To detect the levels of Breg in PBMC and IL-10 and TGF-β expression in seria in cervical carcinoma patients,for evaluating the significance of Breg cells in cervical clinical monitoring.Methods：Br1 subsets in peripheral blood were detected by indirect immunofluorescent staining and the expression level of IL-10 and TGF-β were detected by ELISA in 50 cases with cervical carcinoma.Results：The proportion of Br1 increased in comparison with normal control（P0.05）.The expression levels of IL-10 expression in patients＇ seria were higher than those of control groups（P0.05）;the levels of plasma IL-10 were significantly higher in patients with infiltration as compared with patients with control groups（P0.05）.Conclusion：Predominant expression of IL-10 in cervical cancer patients suggests that Br1 cells may involve in the development of cervical cancer.
关键词
宫颈癌
调节
B细胞
cervical carcinoma
development
Breg
