摘要
目的检测乙肝肝硬化、原发性肝癌患者外周血调节性B细胞(Breg)的比率,了解Breg与肝硬化、肝癌疾病之间的关系。方法以CD19+CD5+CD1d+作为Breg标记,流式细胞术检测24例乙肝肝硬化患者、28例肝癌患者和20名健康对照者外周血Breg占B淋巴细胞的比率。结果肝硬化患者组Breg比率(29.90±9.19)%和肝癌患者组Breg比率(31.34±8.99)%均显著高于健康对照组(22.50±6.52)%,分别为P=0.016和P=0.003;肝硬化患者组和肝癌患者组间Breg比率无统计学意义(P>0.05)。结论与对照组比较,Breg比率在肝硬化、肝癌患者外周血中显著升高,提示Breg细胞增加,与肝硬化、肝癌B细胞免疫调节紊乱相关。
Objective To explore the relationship bewteen regulatory B cells （Breg）with liver cirrhosis and Hepatic carcinomaby analyzing the proportions of Bregs.Methods Taking CD19^＋CD5^＋CD1d^＋ as the marker of the Breg,Flow cytometry was implemented to determine the proportions of Bregs among the B lymphocytes from the 24 patients with liver cirrhosis,28 ones with Hepatic carcinoma and 20 Healthy controls.Results The proportions of Bregs among pa-tients with liver cirrhosis and the ones with Hepatic carcinoma were significantly higher than those of the Healthy con-trols（29.90±9.19% vs 22.50±6.52%,P=0.016 vs P=0.003）,and no significant difference was observed between the proportions among the patients with liver cirrhosis and with Hepatic carcinoma（P〉0.05）.Conclusion The pro-portions of Bregs among patients with liver cirrhosis and Hepatic carcinoma were obviously higher than that of healthy controls which indicates that the increase of Bregs may relate to the regulatory disorders of B lymphocytes in the pa-tients with liver cirrhosis and Hepatic carcinoma.
出处
《中国实验诊断学》
2014年第12期1975-1978,共4页
Chinese Journal of Laboratory Diagnosis
