摘要
[目的]验证黄瓜-酸黄瓜渐渗系群体真实性,获得渐渗系中抗蔓枯病株系。[方法]通过观察统计渐渗系群体的重要农艺性状,利用酸黄瓜特异的SSR标记检测渐渗系材料的真实性,并采用温室苗期人工接种鉴定的方法,对黄瓜-酸黄瓜种间杂交后代(86份渐渗系)群体进行苗期接种鉴定,筛选抗蔓枯病材料。[结果]这些渐渗系在植物学性状上介于两亲本之间,具有部分野生种特有性状,如黑刺、节间短、多分枝等;SSR分子标记能在渐渗系中扩增出野生酸黄瓜的特异DNA片段,从分子水平上证实其为野生酸黄瓜与栽培黄瓜种间杂交后代;从黄瓜-酸黄瓜渐渗系群体中筛选出8份抗性材料,分别为CSIL2、CSIL41、CSIL49、CSIL52、CSIL57、CSIL58、CSIL59、CSIL72,占供试材料的9%。[结论]通过远缘杂交培育渐渗系是拓宽作物遗传的基础,从渐渗系中筛选的8份抗性材料为开展黄瓜抗蔓枯病育种研究提供了抗源材料,同时也为黄瓜抗蔓枯病育种实践奠定了基础。
[ Objectives] The aim of this study is to validate the introgression lines derived from the cross of cucumber and sour cucumber, and obtain materials resistant to gummy stem blight from introgression lines. [ Methods ] Seedlings of 86 introgression lines descended from cucumber-sour cucumber hybrids were subjected to artificial inoculation under greenhouse condition to screen for materials resistant to gummy stem blight. And agronomical traits were observed and analyzed as well. SSR specific to sour cucumber parent were used for confirming the introgression lines. [ Results ] The results showed that the iutrogression lines carryied some sour cucumber morphological traits,such as black spine,short internode and multi-branching. SSR analysis indicated that the introgression lines could amplify sour cucumber specific bands which confirmed these introgression lines containing chromosomal fragments from sour cucumber. Based on these analyses, 8 resistant materials （ CSIL2, CSIL41, CSIL49, CSIL52, CSIL57, CSIL58, CSIL59, CSIL72 ） were selected from the cucumber-sour cucumber introgression lines, accounting for 9% of the tested materials. [ Conclusions ] Introgression lines descended from wide hybridization can be used to broaden the genetic base of crops, and the 8 resistant materials selected from cucumber introgression lines provide resistant resource for genetic research of gummy stem blight and breeding practices.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2015年第3期369-374,共6页
Journal of Nanjing Agricultural University
基金
国家自然科学基金重点项目(31430075)
国家863计划项目(2012AA100102)
教育部‘111’项目(B08025)
国家科技支撑计划项目(2013BAD01B04-10)
江苏省科技支撑计划项目(BE2009310
BE2012323)
国家公益性行业(农业)科研专项(201403032)
关键词
黄瓜
渐渗系
蔓枯病
鉴定
SSR分子标记
cucumber
introgression line
gummy stem blight
identification
SSR markers
