摘要
为研究MyoDⅠ基因在关岭牛不同组织表达量的差异及在肌肉发育过程中的表达情况,试验采用实时荧光定量PCR技术对关岭牛背最长肌、大腿肌、心脏、肝脏、脂肪、小肠6个组织的MyoDⅠ基因相对表达量进行检测。同时分析关岭牛胎儿、18月龄、30月龄时期背最长肌组织中MyoDⅠ基因的表达规律。结果显示,MyoDⅠ基因在背最长肌中表达量最高,在出生后的关岭牛肌肉组织中也具有较高的表达量,且随着出生时间的推移呈上升趋势。推测可能由于背最长肌是肌肉富集地方,因此基因表达量较高,随着年龄增大,肌肉丰满度增加,基因表达量也随着增加。
In order to study differential expression in different tissues and period of MyoD I gene in Guanling cattle. This study detected the expression of MyoD I in longest back muscle, leg muscles,heart,liver, fat and small intestine tissues of Guanling cattle by Real-time quantitative PCR. Meanwhile, analyzed the expressing rules of longest back muscle in foetal, 18 and 30 months. The results showed that the MyoD I was expression at high level in longest back muscle and in muscle tissues after birth had high expression, with the extension of born showed an up-ward trend. It was inferred that the reason of the high expression might be that longest back muscle was muscle enrichment place and increase of muscle fullness.
出处
《中国畜牧兽医》
CAS
北大核心
2015年第4期782-788,共7页
China Animal Husbandry & Veterinary Medicine
基金
国家转基因生物新品种培育重大专项子课题合同书(2013ZX08009-004)
黔科合重大专项字([2013]6008号)
贵州省科技厅农业攻关项目(黔科合NY字[2012]3008号)
贵州大学研究生创新基金(研农2014021)
