摘要
运用DNA池快速测序法寻找牛MyoD1基因SNPs,筛查到1个新的多态位点C1867T,该位点C-T突变引起甘氨酸/丝氨酸的转变。运用PCR-RFLP法验证并分析该位点在不同群体间的多态性,并与相应牛群体的胴体性状进行关联分析。结果显示,中国西门塔尔牛、鲁西牛群体的C1867T位点的突变达到了Hardy-Weinberg平衡状态,而秦川牛未达到平衡状态。不同基因型对肉牛的背膘厚、大腿肉厚的影响差异极显著或显著(P<0.01或P<0.05),对日增重、屠宰率、肌间脂肪、大理石花纹、嫩度的影响差异不显著。
Based on gene pooling and sequencing, a new SNPs was found on the bovine MyoD1 gene, its transition C-T leads to a reaplacement of glicine with serine. Polymorphism and relationship with the carcass traits was analysised by PCR- RFLP in different populations. The transition of this locus fit in with Hardy-Weinberg equilibrium in Chlnese-simmental cattle and Luxi cattle, but not in Qinchuan cattle. Statistical analysis revealed higher value in back fat and thickness of thigh （P〈 0.05）, not in average daily growth, yield of carcass, intramuscular fat, marbling and yenderness.
出处
《中国畜牧兽医》
CAS
2007年第9期40-43,共4页
China Animal Husbandry & Veterinary Medicine
基金
"十一五"国家科技支撑计划重大项目"农林动植物育种工程"(2006BAD01A10)
"十一五"国家高技术研究发展计划(863项目)(2006AA10Z197)资助
