摘要
本研究采用PCR和染色体步移法克隆了草鱼Myo D基因311 bp的内含子1、125 bp的内含子2及长1 066 bp的部分上游启动子序列。同源性比对分析表明,草鱼Myo D的内含子1与鲤科鱼类的同源性相对较高(87%~97.1%)。生物信息学方法预测草鱼Myo D的启动子序列中发现含有1个核心启动子序列,存在CREB、SP1、ATF等多种与转录诱导调控有关的转录因子结合位点,未发现Cp G岛。草鱼Myo D基因内含子及启动子克隆与特征分析,将为进一步研究鱼类Myo D基因的表达调控及其功能分析提供参考。
This study cloned the sequences of Myo D gene introns and its promoters in Ctenopharyngodon idella.PCR and Genome Walking were used to acquire two introns and promoters of the Myo D gene,of which the lengths were 311 bp, 125 bp and 1 066 bp, respectively. Homologous analysis showed that the sequences of the 1 th intron of Ctenopharyngodon idella had higher similarity to the other cyprinidae fish（87%~97.1%）. Bioinformatics methods predicted that one core promoter sequence was found in promoter sequence of Myo D gene in Ctenopharyngodon idella, some transcriptional factor binding sites related to inducible regulation of transcription were found,including CREB, SP1, ATF. Cp G island was not founded. This study would contribute to the further research on the regulation of Myo D expression and the function analysis of Myo D in teleost fish.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2017年第12期5116-5121,共6页
Genomics and Applied Biology
基金
农业部热带亚热带水产资源利用与养殖重点实验室资助
