摘要
目的研究消化复宁汤对肝郁脾虚型慢性萎缩性胃炎(chronic atrophic gastritis,CAG)大鼠细胞外调节蛋白激酶(extracellular regulated protein kinases,ERK)信号通路的影响,探讨消化复宁汤防治CAG的机制。方法将80只雄性Wistar大鼠随机取10只为正常组,其余大鼠随机分为模型组,消化复宁汤高、中、低剂量组和维酶素组。采用脱氧胆酸钠及30%和60%无水乙醇灌胃联合施压束缚和饥饱失常法复制肝郁脾虚证CAG模型。实验结束后,观察各组大鼠一般情况的变化,采用ELISA法检测大鼠血清ERK1/2水平,采用Western Blot法检测大鼠胃组织ERK1/2、p-ERK1/2的表达水平。结果消化复宁汤能明显改善CAG大鼠肝郁脾虚证候表现。消化复宁汤中剂量组与维酶素组血清ERK1/2水平较模型组显著升高(P<0.05),消化复宁汤中、低剂量组与维酶素组大鼠胃组织ERK1/2水平显著高于模型组(P<0.05);消化复宁汤高、中、低剂量组及维酶素组大鼠胃组织p-ERK1/2表达水平较模型组显著降低(P<0.05);消化复宁汤高、中、低剂量组血清ERK1/2水平和胃组织p-ERK1/2表达水平相比较,差异均无统计学意义(P>0.05)。结论消化复宁汤可通过激活ERK1/2,抑制p-ERK1/2的水平防治CAG发生,其作用无明显的剂量依赖性。
Objective To study the effect of Xiaohua Funing Decoction on the extracellular regulated protein kinase（ERK）signaling pathway in rats with liver stagnation and spleen deficiency（LSSD）-type chronic atrophic gastritis（CAG）and to explore the action mechanism of Xiaohua Funing Decoction in the prevention and treatment of CAG.Methods Ten of 80 male Wistar rats were randomly selected as normal group,and other rats were randomly divided into model group,high-,medium-,and low-dose Xiaohua Funing Decoction groups,and vitacoenzyme group.The model of LSSD-type CAG was established by gavage with sodium deoxycholate and anhydrous ethanol（30% and 60%）combined with pressure/bounding and irregular diet.After the experiment was finished,the changes in general conditions of rats were observed in each group.Serum ERK1/2level was measured by ELISA,and the expression of ERK1/2and pERK1/2in gastric tissues was measured by Western blot.Results Xiaohua Funing Decoction significantly improved the LSSD syndrome in rats with CAG.The medium-dose Xiaohua Funing Decoction group and vitacoenzyme group had significantly increased serum ERK1/2levels compared with the model group（P〈0.05）,and the medium-and low-dose Xiaohua Funing Decoction groups and vitacoenzyme group had significantly higher ERK1/2levels in gastric tissues than the model group（P〈0.05）.The high-,medium-,and low-dose Xiaohua Funing Decoction groups and vitacoenzyme group had significantly reduced expression of p-ERK1/2in gastric tissues compared with the model group（P〈0.05）.There were no significant differences in serum ERK1/2level and gastric p-ERK1/2expression between the high-,medium-,and low-dose Xiaohua Funing Decoction groups（P〉0.05）.Conclusion Xiaohua Funing Decoction can prevent and treat CAG by activating ERK1/2and inhibiting p-ERK1/2expression,and its effect is not dosedependent.
出处
《安徽中医药大学学报》
CAS
2015年第2期64-67,共4页
Journal of Anhui University of Chinese Medicine
基金
国家中医药管理局"十二五"全国名老中医药专家传承工作室建设项目
关键词
慢性萎缩性胃炎
消化复宁汤
细胞外调节蛋白激酶
chronic atrophic gastritis
Xiaohua Funing Decoction
extracellular regulated protein kinase
