摘要
目的：建立HPLC同时测定麻花秦艽中马钱苷酸、龙胆苦苷、獐牙菜苦苷、獐牙菜苷、山栀苷甲酯含量的方法。方法：采用Diamonsil C18色谱柱（4.6 mm×250 mm,5μm）,流动相乙腈-0.05%磷酸水梯度洗脱,流速1.0 m L·min-1,柱温30℃,检测波长240 nm。结果：以上5种环烯醚萜类成分的线性范围分别为0.042 1-8.420 0（r=0.999 7）,0.094 2-18.830 0（r=1.000 0）,0.012 4-2.471 2（r=0.999 9）,0.009 9-1.971 2（r=1.000 0）,0.011 3-2.260 0μg（r=0.999 0）,平均回收率均在95.39%-103.75%,RSD≤2.6%。结论：该法简便、快速、准确,可以用来同时测定麻花秦艽中5种环烯醚萜苷成分的含量。
Objective： To establish an HPLC method for the determination of loganic acid, gentiopicroside, swertiamarin, sweroside, shanzhiside methylester in Gentiana straminea. Method： The assay was performed on a Diamonsil C18 column （4.6 mm × 250 mm, 5 μm） eluted with acetonitrile-0.05% phosphoric. The detection wavelength was 240 nm, the flow rate was 1.0 mL ·min^-1, and the column temperature was set at 30 ℃. Result： The calibration curves were linear in the ranges of 0. 042 1-8. 420 0 μg （ r = 0. 999 7） for loganic acid, 0.094 2-18.830 0 μg （r = 1.000 0） for gentiopicroside, 0.012 4-2.471 2 μg （r =0.999 9） for swertiamarin, 0.009 9-1.971 2μg （r = 1.000 0） for sweroside, 0.011 3-2.260 0 μg （r =0.999 0） for shanzhiside methylester. The average recoveries were 95.39%-103.75% （ RSD ≤ 2.6% ）. Conclusion： The method is simple and accurate for the quality control of G. straminea Radix.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第8期77-80,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中央高校基本科研专项重点项目(11NZD02)
四川省科技厅基础研究项目(2015JY0009)
西南民族大学研究生创新型科研项目(CX2014SZ50)
