摘要
采用高效液相色谱法(HPLC)测定甘肃栽培麻花秦艽不同部位、产地和采收期样品的龙胆苦苷含量。结果表明:3年生秦艽的龙胆苦苷含量稍高于2年生,但差异性不显著;根、茎和叶的平均龙胆苦苷含量依次为13.3%、2.95%和2.24%;同一生长年限随采收期延后,茎、叶中龙胆苦苷含量逐渐降低,而根中含量却逐渐上升;不同产地的根样品,依海拔由低到高的,陇西、康乐和临潭分别为10.23%、13.12%和15.54%。本探讨获初步结论:甘肃栽培的麻花秦艽茎和叶的龙胆苦苷均已达到药典的2%标准,宜加以开发利用;从龙胆苦苷产量及生产成本综合考虑,以种植两年较好,根的采收期宜在10月以后封冻前;而以茎叶为生产目标可在8月下旬至9月初采收为佳;高海拔区栽培秦艽有利于秦艽根中龙胆苦苷含量的积累。
Gentiopicrin contents in different organs, habitats and harvest time of Gentiana straminea cultivated at Gansu were determined by high performance liquid chromatography（HPLC）. The results showed that the gentiopicrin contents in the plants growing for three years were slightly higher than those of two years, but the difference was not significant（ P 〉 0. 05 ）. The average gentiopicrin contents in roots, stems and leaves were 13.30% ,2. 95% and 2. 24% respectively. Within the same growing year, gentiopicrin contents were decreased in stems and leaves but increased in roots with the harvest delay. The gentiopierin contents also varied with lower to high ahilude were 10. 23%, 13.12% and 15.54% in Longxi, Kangle and Lintan county respectively. All these results indicated that the gentiopicrin contents in roots, stems and leaves of cultivated G. straminea were all conformable to the Codex criterion（2% ）. Considering both gentiopicrin yield and production cost, the biennial roots should be raked and harvested alter October before soil frozen. However, the harvest should be predated by the end of August or the beginning of September only for stems and leaves. It is benefit for gentiopicrin accumulation in G. straminea when cultivated in high-altitude areas.
出处
《中药材》
CAS
CSCD
北大核心
2007年第10期1214-1216,共3页
Journal of Chinese Medicinal Materials
基金
甘肃省科技扶贫项目
甘肃省科技攻关项目(2GS042-A43-013-062GS064-A43-019-08)
甘肃省自然科学基金项目(9560433)
兰州市科技局项目(06-2-60)
关键词
麻花秦艽
龙胆苦苷
甘肃
栽培
Gentiana straminea Maxim.
Gentiopicrin
Gansu
Cultivation
