摘要
环烯醚萜类成分是中药秦艽的主要活性成分,其中马钱苷酸、6'-O-β-D-葡萄糖基龙胆苦苷、獐牙菜苦苷、龙胆苦苷、獐牙菜苷在秦艽药材中的含量相对较高且具有良好的药理活性。实验中建立了中药秦艽中5种环烯醚萜的含量测定方法:Shiseido SPOLAR C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相为0.04%甲酸水(A)-乙腈(B)梯度洗脱;体积流量0.8 mL·min^(-1);检测波长240 nm;柱温30℃;进样量5μL。该方法分离度良好,标准曲线在线性范围内相关性良好,加样回收率均值在97.35%~106.2%。对8种39份秦艽药材进行含量测定,结果显示5种环烯醚萜类成分在8种秦艽类药材中均有分布。种内含量差异较大表明含量除了与物种有关外,与种质资源、环境和生长年限都密切相关。小秦艽中各组分的含量明显低于其他3种正品秦艽。
This study aims to establish a new method for quality evaluation of Gentianae Macrophyllae Radix by simultaneous determination of five iridoids （loganic acid, 6＇-O-β-D-glucopyranosylgentiopicroside, swertiamarin, gentiopieroside, sweroside）, and to detect five iridoids in the root of eight species （ Gentiana rnacrophylla, G. straminea, G. crassicaulis, G. dahurica, G. robusta, G. waltonii, G. lhassica, and G. tibetica）. The separation was carried out on a Shiseido SPOLAR C18 （4. 6 mm × 250 mm,5 um） column eluted with mobile phase of water containing 0. 04% formic acid （A） and acetonitrile （B） in a gradient program. The flow rate was 0. 8 mL . min-1. The detect wavelength was set at 240 nm. The column temperature was kept at 30℃. The volume of injection was 5 uL. The five iridoids were well separated with ideal linear correlations. The average recoveries were 97. 35% -106. 23%. All the five iridoids were detected in the root of eight species. The contents of same species changed in a somewhat wider range. The contents in root of G. dahurica were lower than that in other species.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第4期715-720,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81173654)
上海市科委一般项目(10ZR1429800)
关键词
秦艽
HPLC
环烯醚萜
Gentianae Macrophyllae Radix
HPLC
iridoids
