摘要
目的建立HPLC测定秦艽总苷提取物中龙胆苦苷的含量。方法采用HPLC法,以C18柱为色谱柱,以甲醇-水(25∶75)为流动相,检测波长为272nm,流速为1.0mL.min-1。结果龙胆苦苷在0.657～3.942μg范围内线性关系良好,相关系数为0.999 3;平均回收率为98.14%,RSD为0.87%。结论该方法简便、准确,结果可靠,可用于秦艽总苷提取物的质量控制。
Objective To establish a quantitative method for determination of gentiopicroside in the total iridoid glucosides（TIG） extracted from Gentiana macrophylla Pall. Methods HPLC analysis was performed on a Hypersil C18 column （250 minx 4.6 mm, 5 pm） with isocratic elution,methanol-water {25 ： 75） as the mobile phase at a flow-rate of 1.0 mL · min-1 ,with detection at 272 nm. Results The regression equations revealed good linear relationships between the ratios of peak-area to concentrations. Gentiopicroside was good linearity in the range of 0. 657-3. 942 btg（r=0. 999 3） ,and the average recovery was 98.14% ,with RSD 0.875（n=6）. Conclusion The method was simple,accurate,reliable,and suitable for the quantitative evaluation of gentiopicroside in the TIG of Gentiana macroph vlla Pall.
出处
《西北药学杂志》
CAS
2012年第2期106-108,共3页
Northwest Pharmaceutical Journal
基金
国家科技重大专项(编号:2011ZX09401-308-036)
陕西教育厅项目(编号:09JK388)
陕西省"13115"创新工程项目(编号:2009ZDKG-81)
关键词
秦艽总苷提取物
高效液相色谱法
龙胆苦苷
TIG extracted from Gentiana macrophylla Pall.
HPLC
gentiopicroside
