摘要
目的:通过观察大鼠腹腔灌注5%Na HCO3后CO2气腹对腹腔液p H及TNF-α的影响,探讨其效果及机理,为临床腹腔镜手术治疗恶性肿瘤提供理论依据。方法:选取健康Wistar大鼠30只,分为3组。实验组:以10 ml 5%Na HCO3溶液灌洗腹腔,建立CO2气腹;气腹组:维持动态CO2气腹90 min;对照组:仅行单纯麻醉,不建立气腹。30 min时,实验组于腹腔内注入10 ml生理盐水灌洗,气腹组、对照组注入20 ml。分别于30 min、60 min、90 min、24 h、48 h、72 h取腹腔液样本,测定各组腹腔液p H值,酶联免疫吸附试验法测定TNF-α含量。结果:30 min、60 min、90 min时,实验组p H值明显高于气腹组,差异有统计学意义(P<0.01);24 h后两组差异无统计学意义(P>0.05)。30 min、60 min、90 min、24 h后,实验组TNF-α明显高于气腹组,差异有统计学意义(P<0.05);48 h、72 h时,两组差异无统计学意义(P>0.05)。结论:大鼠腹腔灌注5%碳酸氢钠溶液后可提升术中腹腔局部微环境p H值,保护了CO2气腹腹腔镜手术后腹膜巨噬细胞产生TNF-α的功能,可能会减少肿瘤腹腔内播散及穿刺孔转移的发生。
Objective：Through the method of intraperitoneal infusion of 5% Na HCO3 into abdominal cavity of rats,the study is to observe the influence of CO2 pneumoperitoneum on peritoneal fluid p H and TNF-α,to evaluate its effectiveness,and to explore its mechanism,in order to provide a theoretical basis for the clinical laparoscopic surgery in the field of malignancy treatment. Methods：Thirty healthy Wistar rats were selected and divided into three groups. Experimental group：10 ml of 5% Na HCO3 solution was applied to the rats for intraperitoneal lavage to establish the CO2 pneumoperitoneum. Pure CO2 pneumoperitoneum group：dynamic CO2 pneumoperitoneum maintained for 90 min. Each group drained liquid after 90 min. The control group was only performed simple anesthesia without pneumoperitoneum. After 30 min,10 ml of 0. 9% Na Cl solution was applied to the rats of experimental group for intraperitoneal lavage and 20 ml of 0. 9% Na Cl solution was given to the pure CO2 pneumoperitoneum group and the control group respectively. After30 min,60 min,90 min,24 h,48 h and 72 h,peritoneal fluid samples were taken to observe the p H value of the peritoneal fluid. The enzyme-linked immunosorbent assay method was used for the measurement of the TNF-α. Results：p H value：after 30 min,60 min and90 min,the value of the experimental group was significantly higher than that in the CO2 pneumoperitoneum group,and the difference was statistically significant（ P〈0. 01）;there was no significant difference after 24 h（ P〉0. 05）. TNF-α：after 30 min,60 min,90 min and 24 h,for the experimental group and CO2 pneumoperitoneum group,the TNF-α produced from the experimental group was significantly higher than that from CO2 pneumoperitoneum group and the difference was statistically significant（ P〈0. 05）;After 48 h and72 h,there was no significant difference between two groups（ P〉0. 05）. Conclusions：Peritoneal infusion of 5% sodium bicarbonate solution into rats may increase the p H value in abdominal cavity micr
出处
《腹腔镜外科杂志》
2015年第2期107-111,共5页
Journal of Laparoscopic Surgery
关键词
碳酸氢钠
二氧化碳
气腹
腹腔液
p
H
肿瘤坏死因子Α
Sodium bicarbonate
Carbon dioxide
Pneumoperitoneum
Peritoneal fluid
p H
Tumor necrosis factor-alpha
