摘要
目的:探讨腹腔镜不同气腹介质对人宫颈癌细胞株CaSki体外生长的影响。方法:体外培养CaSki细胞,模拟腹腔镜建立CO2气腹和N2气腹处理细胞,另设未处理组为对照。采用MTT法、流式细胞术及电镜比较各组CaSki细胞的生长情况。结果:处理后48h起CO2促进CaSki细胞生长,N2组在处理后48￣72h短暂抑制细胞增殖;CO2组G2-M期细胞明显增加;CO2组细胞见明显核分裂像,N2组可见线粒体肿胀,核周间隙扩大。结论:CO2气腹促进CaSki细胞生长,而N2气腹则起一定的抑制作用。
Objective ：To evaluate the effects of different used in laparoscopy on the growth of human cervical carcinoma cells in vitro.Methods：Human cervical carcinoma cell line （CaSki）were cultured in vitro and then exposed to pneumopefitoneums of pure carbon dioxide or pure nitrogen gas with the untreated group as control. The cell growth was determined by MTT assay,flow cytometry and electron microscopy. Results：Carbon dioxide stimulated the cell growth from 48h to 120h after treatment. For nitrogen gas group,there was a transient descent of cell proliferation from 48h to 72h after treatment. The tumor cells of carbon dioxide group in G2-M phases significantly increased. Active karyokinesis was observed in carbon dioxide group while slight mitochondrion swelling was observed in nitrogen gas group with the nucleus space expanded. Conclusions： Carbon dioxide pneumoperitoneum could increase the growth of human cervical carcinoma cells in vitro whereas nitrogen gas pneumoperitoneum had inhibiting effect on the cell growth.
出处
《重庆医科大学学报》
CAS
CSCD
2007年第3期261-263,321,共4页
Journal of Chongqing Medical University
关键词
腹腔镜
二氧化碳
N2
气腹
宫颈肿瘤
体外生长
Laparoscopy
Carbon dioxide
Nitrogen gas
Pneumoperitoneum
Cervical neoplasm
Cell growth in vitro
