摘要
目的:观察益气活血方对心力衰竭大鼠心肌能量代谢的影响。方法:大鼠80只,经冠脉结扎法配合力竭式游泳、饥饿等方法造成心衰模型,手术成功2周后,将存活大鼠分为模型组、中药组、西药组,另设正常组共4组,均以灌胃给药或蒸馏水28d。利用蛋白免疫印迹法(Westem blot)和RT-PCR法分别检测过氧化物酶体增殖物活化受体γ辅助激活因子-1(PPARγ-coactivator-1,PGC-1)的蛋白和基因表达的变化情况,比色法测定高能磷酸盐(adenosine triphosphate,ATP)浓度,ELISA法测定血清中N末端脑钠肽前体(N-terminal pro-brain natriuretic peptide,NT-proBNP)及心肌肌钙蛋白I(troponin I,cTNI)的浓度。结果:与正常组相比,模型组及余各组大鼠ATP及PGC-1α水平显著降低(P<0.01),NT-proBNP及cTnI含量显著升高(P<0.01);与模型组相比,中药组和西药组ATP及PGC-1α明显升高(P<0.01),NT-proBNP及cTnI含量明显降低(P<0.01);西药组与中药组血清中ATP,PGC-1α,NT-proBNP,cTnI含量无明显差异。结论:益气活血中药可显著降低心衰大鼠的NT-proBNP及cTnI含量,改善能量代谢,促进PGC-1α生成,延缓慢性心衰的发展。
Objective: The aim of this article was to observe the effect of Yiqi Huoxue decoction on myocardial energy metabolism of rats with congestive heart failure. Method: Eighty rats were subject to exhaustive swimming and hunger to cause heart failure model with coronary artery ligation. Two weeks after operation, the survivals were divided into four groups: model group, Chinese medicine group, western medicine group, and normal group 4 group, treated by gavage or with distilled water for 28 days. We detected the changes of the expression of proteins and genes of peroxisome proliferator activation receptor gamma coactivator 1 (PPAR -/-coaetivator-1, PGC-1 ) by using Western blot and RT-PCR method. The coneentration of high-energy phosphate ( adenosine triphosphate, ATP) was determined by using colorimetric method, and the contents of serum N terminal pro brain natriuretic peptide (NT proBNP) and cardiac troponin I (cTNI) concentration were determined via ELISA. Result: Compared with normal group, the ATP and PGC-1 a levels of rats in model group and other groups were significantly lower (P 〈 0.01 ) , while the contents of NT-proBNP and eTnI were significantly increased (P 〈 0.01 ) ; compared with the model group, the ATP and PGC-1 c- levels of rats in Chinese medicine group and western medicine group, increased significantly (P 〈 0. 01 ), the contents of NT-proBNP and cTnI decreased (P 〈 0.01 ). In western medicine group and Chinese medicine group, there was no significant difference inthe levels of ATP, PGC-1 α, NT-proBNP and cTnI in the serum. Conclusion: Yiqi Huoxue decoction can significantly reduce NT-proBNP and cTnI content in rats with chronic heart failure, improve the energy metabolism, promote the production of PGC-1 alpha, and delay the development of chronic heart failure.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第6期169-173,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
沈阳市科技计划项目(F13-316-1-10)