摘要
目的建立测定甜梦胶囊中黄芪甲苷含量的高效液相色谱方法。方法色谱柱用Zorbax Eclipse XDB-C18柱（150 mm×4.6 mm,5μm）,流动相为乙腈-水（32∶68）,流速为1.0 m L/min。结果黄芪甲苷进样量的线性范围为0.230 4~4.608μg（r=0.999 7）,平均回收率为98.15%,RSD为1.46%（n=6）。结论该方法用于测定甜梦胶囊中黄芪甲苷含量专属性强、操作简便。
Objective To establish a HPLC-ELSD method for the determination of the astragaloside content in Tianmeng Capsules.Methods The ZORBAX Eclipse XDB-C18 column（ 150 mm × 4. 6 mm,5 μm） was used with acetonitrile-water（ 32 ∶ 68） as the mobile phase. The flow rate was 1. 0 m L / min. Results The linear range of astragaloside was 0. 230 4-4. 608 μg（ r = 0. 999 7）. The average recovery rate was 98. 15% with RSD of 1. 46%（ n = 6）. Conclusion This method is strongly specific and simple to operate for detecting the astragaloside content in Tianmeng Capsules.
出处
《中国药业》
CAS
2015年第5期31-32,共2页
China Pharmaceuticals
