摘要
以13年生苏玛栎优树环割萌蘖的半木质化枝条做为外植体,采用离体培养的方法建立了苏玛栎植株再生体系,结果表明:在WPM培养基上添加BA1.0 mg·L-1、NAA 0.1 mg·L-1和生物素5 mg·L-1、乙酰胆碱5 mg·L-1、核黄素5 mg·L-1时,培养40 d,增殖系数为2.15;单芽在1/2WPM+NAA2.0 mg·L-1,添加生物素5 mg·L-1、乙酰胆碱5 mg·L-1、核黄素5 mg·L-1的培养基上生根率为10%,采用试管外生根,生根率可达到80%。本研究建立了苏玛栎离体培养和植株再生技术体系,为推广苏玛栎良种奠定坚实基础。
With semi-lignified shoots sprouted from the girdling of 13 years old Quercus shumardii as explants,we established the regeneration system of Q. shumardii by in vitro culture. When the WPM was supplemented with 1. 0 mg· L- 1BA,0. 1 mg · L- 1NAA and 5 mg · L- 1Biotin,5 mg · L- 1acetylcholine,5 mg · L- 1vitamine B2,and cultured for 40 d,the multiplication coefficient was 2. 15. The rooting rate of single bud was10% when cultured in 1 /2 WPM supplemented with 2. 0 mg · L- 1NAA,5 mg · L- 1Biotin,5 mg · L- 1acetylcholine,5 mg·L- 1vitamine B2. The rooting rate was about 80% when roots were induced out-tube. Our work established technical system for tissue culture and plantlet regeneration of Q. shumardii with a solid foundation for the extension of its good quality seedlings.
出处
《植物研究》
CAS
CSCD
北大核心
2015年第2期185-190,共6页
Bulletin of Botanical Research
基金
上海市科技兴农重点攻关项目"优良栎类树种单株选优和扩繁技术研究"[沪农科攻字(2009)第9-5号]资助
关键词
苏玛栎
离体培养
植株再生
Quercus shumardii
in vitro culture
plantlet regeneration
