摘要
以成龄(30年以上)栓皮栎枝条的萌条在室内沙培催芽,用萌芽作为外植体建立无菌苗体系,研究了基本培养基、蔗糖浓度以及IBA浓度和处理时间对生根的影响。结果表明:采用1/4MS培养基添加30g/L蔗糖为较适宜的培养基,生根率达到了66.4%,平均生根数为2.3条,平均根长为4.82 cm。采用两步生根法,用添加1.0mg/L的IBA培养基处理14天,然后转接到无激素的培养基上的生根率最高,达到了96.7%,愈伤组织少。
Accelerating the germination of branches obtained from over 30 years old quercus variabilis trees by indoor sand culture, then using coppice sprouts from the branches as explants to build tissue cultural system, this experiment studies the effect of the cultural media kinds,the sucrose concentration,the IBA concentration and the processing time on the ratio of rooting. The result shows that 1/4MS with 30g/L sucrose is the best medium, the rooting ratio is up to 66.4%, the average number of the rooting is 2.3, and the average length of the roots is 4. 82cm; following the two -step rooting method, the shoots have been cultured by adding 1.0mg/L IBA for 14 days, then transferred to the medium without hormone. In this way, a high rooting ratio is up to 96.7%, and there is no callus in the base.
出处
《绵阳师范学院学报》
2006年第5期79-82,95,共5页
Journal of Mianyang Teachers＇ College
基金
绵阳师范学院青年教师科研基金项目(MA2005021)
关键词
拴皮栎
组培
生根
影响因素
quercus variabilis
tissue culture
rooting
influencing factors
