摘要
目的:研究在机械力作用下人牙周膜细胞(h PDLCs)内Periositn(PN)m RNA和蛋白的表达变化。方法:采用组织块酶消化法培养h PDLCs,经鉴定后将第4代细胞随机分为4个实验组和1个对照组(非加力组),4个实验组分别采用Flexcell-5000 Tension System应力加载系统对h PDLCs加载6、12、24、48 h的周期性张应力;然后采用q RT-PCR、Western blot分别检测各组h PDLCs中PN m RNA和蛋白的表达变化。结果:在正常h PDLCs中表达PN m RNA和蛋白;与对照组相比,加力6 h后,PN m RNA和蛋白的表达水平均明显降低(P<0.05);加力12 h后,PN m RNA和蛋白的表达均逐渐升高,并持续至24 h达最高水平(P<0.05);而在加力48 h时,PN m RNA和蛋白表达均明显下降,并恢复至对照组的表达水平(P>0.05)。结论:机械力可诱导h PDLCs中PN的表达增强及活化,且具有时间依赖性;提示PN可能参与了细胞内生物力学信号的转导。
AIM： To observe the effects of cyclic stretch on the expression of periositn（ PN） in human periodontal ligament cells（ h PDLCs）. METHODS： Primary h PDLCs were cultured by tissue block enzymolytic method.Cells were subjected to cyclic stretch by Flexcell FX-5000 T system at 0. 5 Hz of 10% elongation for 6,12,24 and 48 hours respectively. The m RNA and protein expression of PN were measured by q RT-PCR and western blot respectively. RESULTS： PN m RNA and protein were positively expressed in control h PDLCs. PN m RNA and protein were decreased after 6-hour cyclic stretch,then increased after 12- hour cyclic stretch and reached to the highest level after24-hour treatment（ P〈0. 05）. After 48 hour treatment,PN expression began to decline,consistent with the expression level of the control cells. CONCLUSION： Cyclic stretch may change the expression level of PN in h PDLCs in a time-dependent manner. PN may plays an important role in cell signal transduction of mechanical strain.
出处
《牙体牙髓牙周病学杂志》
CAS
2015年第2期79-83,共5页
Chinese Journal of Conservative Dentistry
基金
广东省高等学校人才引进科研资助项目(C1030270)
