摘要
以平菇、香菇、灵芝、真姬菇、银耳、杏鲍菇、金针菇7种常见菌草菌糠浸提液配制的培养基为试材,研究了其对竹荪菌丝生长的影响,进而对最适竹荪菌丝生长的菌糠进行水溶物质的提取。将该菌糠提取物配制的不同浓度梯度的培养基与PDA培养基进行对比,筛选出最适的菌糠提取物添加量,旨在研究出一种新式竹荪母种培养基。结果表明:以菌草灵芝菌糠浸提液作为培养基,竹荪菌丝生长速度快于其它菌糠与PDA培养基(P<0.01)。当向母种培养基中添加6g/L菌草灵芝菌糠提取物时,最适于竹荪菌丝的快速生长,且菌丝粗壮、洁白。
Spent mushroom substrate （SMS） from Pleurotus ostreatus ,Lentinus edodes ,Ganoderrna lucidum , Hypsiz ygus rnarmoreus, Tremella fuciJbrmis, Pleurotus eryngii and Flarnrnulina velutipes that cultivated by Juncao technology were used for the materials selection in producing mother culture media for the cultivation of D. indusiata mycelium. Water- soluble substance of SMS that can promote the rapid growth of D. indusiata mycelium were extracted. Then, culture media added with different ratio of water-soluble extracts were used in growing mycelium of D. indusiata, and then the appropriate dose of SMS extracts were screened in order to get a new formula of mother cultrue media of D. indusiata, compared with general PDA media. The results showed that water-soluble extracts from SMS of G. lucidum performed the best effect in growth of D. indusiata mycelium, and the latter grew faster compared with other spent mushroom substrates （P〈0. 01）. D. indusiata mycelium grew faster and showed better quality while 6 g extracts from SMS G. lucidum were added to 1 L mother media.
出处
《北方园艺》
CAS
北大核心
2015年第5期155-159,共5页
Northern Horticulture
基金
福建省科技重大专项资助项目(2012NZ0002)
科技部国家中心组建资助项目(2011FU125X11)
关键词
菌草
菌糠
提取物
竹荪
母种培养基
Juncao
spent mushroom substrate
extracts
D.indusiata
mother culture media
