摘要
目的探讨中药百令胶囊（BL）对病毒性心肌炎（VMC）小鼠心肌组织骨膜蛋白表达及心肌纤维化的影响，阐明其抗心肌纤维化的可能机制。方法以柯萨奇病毒B，（CVB，）感染50只BALA／c小鼠建立VMC心肌纤维化动物模型，同时设对照组（10只），建模好的31只小鼠按随机数字表法分为模型组（11只）、大剂量BL组（10只）和小剂量BL组（10只）。模型组和对照组小鼠给予自来水1mL／d，大剂量BL组小鼠给予BL7．5g/（k·d），小剂量BL组给予BL2．5g／（kg·d），60d后ELISA法测各组小鼠血清血管紧张素Ⅱ（AngⅡ）水平，对4组小鼠的心脏切片行Masson染色计算心肌胶原容积分数（CVF），应用反转录聚合酶链反应（RT—PCR）法对转化生长因子β1（TGF—β1）、骨膜蛋白在小鼠心肌组织中的表达水平行半定量分析。结果与对照组比较，模型组小鼠的血清AngⅡ水平、CVF、TGF-β1mRNA和骨膜蛋白mRNA表达均显著升高（P均〈0．01）；与模型组比较，大剂量BL组和小剂量BL组血清AngⅡ水平、CVF、TGF—β1mRNA表达水平均下降（P均〈0．05）。与模型组比较，大剂量BL组心肌骨膜蛋白mRNA表达水平下降（P〈0．05），小剂量BL组心肌骨膜蛋白mRNA表达无明显下降（P〉0．05）。结论BL具有抗VMC小鼠心肌纤维化作用，其作用机制可能是通过调控血清AngⅡ水平，抑制心肌TGF-β1表达，进而减少骨膜蛋白表达水平而实现的。
Objective To explore the preventive effect of Bailing capsules（BL） on myocardial fibrosis in the viral myocarditis（VMC） mice models and to clarify the possible mechanism. Methods The models of VMC were established by injecting Coxsackie virus B3 （ CVB3 ） solution into 50 BALA/C mice. The 31 mice were divided into model group（n =11 ）,large dose BL group（n = 10）and small dose BL group （n = 10）. At the same time the control group （n = 10） was established. The mice in model group and control group were fed with water 1 mL/d,the mice in large dose BL group were fed with BL at a dose of 7.5 g/（kg· d） ,and the mice in small dose BL group were fed with BL at a dose of 2.5 g/（ kg · d）. After 60 d,the levels of angiotensin Ⅱ （ Ang Ⅱ ） in blood serum of mice in various groups were detected by using ELISA. Myocardium tissue of mice was stained by Masson and collagen volume fraction（CVF） was accounted. At the same time,the content of periostin mRNA and transforming growth factor β1 （ TGF - β1 ） mRNA in myocardium tissue of mice were detected by adopting reverse transcription（RT） - PCR. Results Compared with the control group, the levels of serum Ang Ⅱ , CVF, TGF - β1 mRNA and periostin mRNA were increased （ all P 〈 0. 01 ） in the model group. Compared with the model group, the levels of serum Ang Ⅱ , CVF and TGF - β1 mRNA were obviously decreased in large dose BL group and small dose BL group （ all P 〈 0.05 ）. Compared with the model group, the level of periostin mRNA was obviously decreased in large dose BL group （ P 〈 0.05 ）, but the level of periostin mRNA in small dose BL group was not decreased obviously （P 〉 0. 05）. Conclusions BL can offer some protection to myocardial fibrosis in VMC mice. The possible mechanism may be performed through inhibiting the expression of serum Ang Ⅱ and TGF- β1 in myocardium tissue to reduce the expression of periostin.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2015年第1期64-67,共4页
Chinese Journal of Applied Clinical Pediatrics
基金
基金项目：吉林省科技厅重点科技攻关项目（20140204041）
