摘要
目的探讨白细胞介素-17A（IL-17A）在病毒性心肌炎（VM）小鼠中的表达及其临床意义。方法将55只Balb／c小鼠按随机数字表法分为心肌炎组（45只）和对照组（10只），心肌炎组腹腔接种0．1mL含柯萨奇B3病毒（CVB3）的Eagle’S液建立VM动物模型，分别于接种后第7、14、28天随机取10只处死，对照组腹腔接种0．1mL不含病毒的Eagle’s液，于第28天处死，运用苏木精-伊红（HE）染色检查心肌病理改变，实时荧光定量PCR（RQ—PCR）、Westernblot测定心肌IL-17AmRNA、蛋白表达，ELISA法检测血清IL-17A水平，并对心肌炎组心肌IL-17A蛋白表达水平、血清IL-17A水平与心肌病变积分进行相关性分析。结果心肌炎组接种病毒后第7天、第14天心肌IL-17AmRNA及蛋白表达水平、血清IL-17A水平均显著高于对照组（P均〈0．01），并且心肌炎组心肌IL-17A蛋白表达水平、血清IL-17A水平与心肌病变积分呈正相关（r=0．67、0．74，P均〈0．05）。结论IL．17A可能在VM的发病机制中起重要作用，且与VM的病情严重程度有关，可作为VM新的检测指标。
Objective To explore the expression of interleukin-17A（IL-17A） in the myocardium of mice with experimental viral myocarditis （VM） and its clinical significance. Methods Fifty-five mice were randomly divided into myocarditis group（ n = 45 ） and control group（ n =10）. Mice in the myocarditis group were inoculated intraperitoneally with 0.1 mL Eagle＇s solution containing coxsackievirus B3 （CVB3） to establish VM models. However, mice in the control mice were treated with 0.1 mL Eagle＇s solution. Ten infected mice were sacrificed on day 7,14 and 28 after inoculation, and the control mice were killed on day 28 postinoculation. Myocardial histopathology was determined with hematoxylin and eosin stain. The levels of myocardial IL-17A mRNA and protein expressions were detected by real time quantitative polymerase chain reaction（RQ-PCR） and immunohistochemistry. Serum IL-17A concentration was examined using enzyme linked immunosorbent assay（ELISA）. In addition,the relationship between myocardial IL-17A protein levels as well as serum IL-I7A concentration and myocardial histopathological scores were analyzed statistically in myocarditis group. Results The levels of myocardial IL-17A mRNA and protein and serum IL-17A concentration on day 7 and 14 after inoculation in myocarditis group increased significantly compared with those in control group（ all P 〈 0.01 ）. Myocardial IL-17A protein levels and serum IL-17A concentration showed significantly positive correlation with the myocardial histopathologie scores in VM group, respectively （ r = 0.67,0.74, all P 〈 0.05 ）. Conclusions IL-17A may play a pivotal role in the pathogenesis of VM and is associated with the severity of VM. It can serve as a novel indi- cator for VM.
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2014年第1期45-47,共3页
Chinese Journal of Applied Clinical Pediatrics
