摘要
目的研究二乙基亚硝胺(DEN)诱导的小鼠肝细胞癌(HCC)过程中解整合素-金属蛋白酶8(ADAM8)表达的动态变化。方法健康雄性BALB/c小鼠70只分为正常对照组和实验组,实验组小鼠每日喂予30μg·m L-1DEN,连续诱癌24周。在诱癌后4,8,12,16,20和24周,分别处死10只小鼠,取肝脏,分为2份,一份用HE染色法检测肝癌的发生情况,另一份用蛋白印迹法检测小鼠肝脏ADAM8蛋白的表达情况。对正常小鼠处理方法相同。结果 DEN诱导小鼠肝细胞癌24周诱癌成功,ADAM8在小鼠诱癌过程中表达量逐渐增加,在诱癌后24周表达量达到最高值。结论 ADAM8在肝细胞癌发生发展过程中表达呈显著变化,对肝细胞癌的发生和发展可能起到重要的促进作用。
Objective To study the expressed dynamic change of a dis-integrin and metalloprotease 8 （ ADAM8 ） during hepatocellular carci-noma （ HCC ） induced by diethylnitrosamine （ DEN ） in mice.Methods Seventy mice were randomly divided into two groups：nor-mal group （ n=10 ） and experimental group （ n=60 ）.The mice in experimental group were fed daily with 30 μg . mL -1 DEN in drin-king water for 24 weeks.Ten mice were respectively sacrificed at 4 , 8 , 12 , 16 , 20 and 24 weeks, then the livers were taken from the mice and divided into two sections.One was used to detect the occurrence of liver cancer by HE staining, the other was used to detect the ADAM8 expression by Western blot during HCC induced by DEN in mice.The normal control mice were also detected as experimental mice.Results HCC model was successfully induced at 24 weeks after DEN induction.The expression of ADAM8 was gradually increased and reached the peak level at 24 weeks after DEN induc-tion.Conclusion ADAM8 were remarkably differently expressed during HCC induced by DEN in mice.ADAM8 could play important roles in promoting the occurrence and development of HCC.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2014年第12期1105-1107,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81201558)
河南省高校科技创新人才支持计划基金资助项目(13HASTIT025)
河南省重点科技攻关基金资助项目(122102310030)
河南省教育厅科学技术研究基金重点资助项目(12B310003)
