摘要
目的研究增殖细胞核抗原(PCNA)在二乙基亚硝胺(DEN)诱导的小鼠肝细胞癌中表达的变化及意义。方法小鼠用含DEN的饮水连续诱癌24周。在诱癌后不同时间点,处死小鼠,分别用蛋白印迹法和逆转录聚合酶链反应检测小鼠肝PCNA蛋白与核酸的表达。结果 DEN诱癌后,小鼠肝组织中PCNA蛋白与核酸的表达逐渐增高,在第24周达高峰,差异有统计学意义(P<0.05)。结论在DEN诱导的小鼠肝细胞癌过程中,PCNA的表达出现显著的动态变化,说明它可能在肝细胞癌的发生发展过程中起到重要作用。
Objective To investigate the dynamic change and signifi-cance of proliferating cell nuclear antigen （ PCNA ） expression of mice which were induced into hepatocellular carcinoma （ HCC ） by diethylni-trosamine （ DEN ） .Methods Mice continuously induced cancer with DEN containing drinking water for 24 weeks .The mice were killed in the induction of cancer at different time points.The expression of PCNA protein and nucleic acid in mice liver with HCC were evaluated by Western blot and reverse transcription polymerase chain reaction,respec-tively.Results The expressions level of PCNA protein and nucleic acid in the liver tissue in mice after induced into HCC by DEN increased gra-dually.They reached a peaked at 24th week, and there was significant differences between them （ P〈0.05 ）.Conclusion There is a signifi-cant dynamic change of PCNA expression in the liver tissue in mice during the progression of HCC which were induced by DEN.It suggest that PCNA might play an important role in the incidence and development of HCC.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第9期738-740,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81201558)
河南省高校科技创新人才支持计划基金资助项目(13HASTIT025)
河南省重点科技攻关基金资助项目(122102310030)
河南省教育厅科学技术研究基金重点资助项目(12B310003)
关键词
肝细胞癌
增殖细胞核抗原
二乙基亚硝胺
hepatocellular carcinoma
proliferating cell nuclear antigen
diethylnitrosamine
