摘要
目的探讨血清和肿瘤组织可溶性间皮素相关蛋白(SMRP)和糖类抗原125(CA125)的表达水平对卵巢癌的临床意义。方法用酶联免疫吸附测定(ELISA)检测82例卵巢癌患者(A组)、76例卵巢良性肿瘤组患者(B组)和53例健康妇女(C组)的血清CA125和SMRP水平,免疫组化检测卵巢组织中CA125和SMRP水平。1年后测定卵巢癌的血清CA125和SMRP水平,分析各统计指标的相关性。结果 A组CA125和SMRP阳性表达率明显高于B组(P<0.05)。相对于B组和C组,A组术前血清中CA125和SMRP水平较高(P<0.05);B组术前血清中CA125水平高于C组(P<0.05);与Ⅰ、Ⅱ期比较,卵巢癌Ⅲ、Ⅳ期术前患者的血清CA125和SMRP水平较高(P<0.05);与术前比较,术后CA125和SMRP水平较低(P<0.05)。出院1年后,与基本稳定患者相比,卵巢癌组中复发的患者血清CA125和SMRP水平明显上升(P<0.05)。对于诊断卵巢癌的敏感性和特异性,任何一项单独检测都不如结合血清CA125和SMRP检测。结论 CA125、SMRP的联合检测对提高卵巢癌诊断的敏感性和特异性、早期诊断、病情监测与疗效评估具有重要价值。
Objective To explore the clinical significance of expression of soluble mesothelin retatedprotein（SMRP） and carbohydrate antigen （CA125） in the serum and tumor tissues of the patients with ovarian cancer. Methods The preoperative and postoperative levels of SMRP and CA125 in serum and ovarian cancer tissues were detected in 82 patients with ovarian cancer （group A） ,76 cases of benign ovarian tumor （group 13） and 53 healthy women （group C） by using ELISA and the immunohistochemical method respectively. Serum levels of SMRP and CA125 in the ovarian cancer patients were measured after one year by ELISA. The correlation among the various statistical indexes was analyzed. Results The positive expression rates of SMRP and CA125 in the group A were significantly higher than those in the group B（P〈0. 05）;compared with the group B and C, the preoperative serum level of CA125 and SMRP in the group A was significantly increased （P〈0. 001） ;the preoperative serum CA 125 level in the group B was higher than that in the group C;compared with the stage Ⅰ and Ⅱ,the serum CA125 and SMRP in the stage Ⅲ and Ⅳ of o- varian cancer were significantly increased （ P〈0.05） ; compared with before operation, the postoperative SMRP and CA125 levels were significantly decreased（P〈0.05）. After 1 year of discharge from hospital, compared with the basically stable patients, serum CA125 and SMRp levels in the patients with ovarian cancer recurrence were significantly increased（P〈.0.05）. The sensitivity and specificity for diagnosing ovarian cancer,any single detection was inferior to the combination detection of CA125 and SMRP. Conclu- sion The combination detection of CA125 and SMRP has an important value for increasing the sensitivity and specificity of ovarian cancer diagnosis, early diagnosis, illness condition monitoring and effect evaluation.
出处
《国际检验医学杂志》
CAS
2015年第1期22-24,共3页
International Journal of Laboratory Medicine
基金
上海市卫生局科研项目(2011208)
