摘要
以野生樟叶越桔幼嫩带芽茎段为外植体,通过对初代启动培养、增殖及生根培养方案的筛选,建立了樟叶越桔的组织培养和快速繁殖体系。结果表明,最适启动培养基为WPM+6-BA 3.0 mg·L-1+NAA 0.2 mg·L-1,外植体诱导萌发率达83.33%;增殖培养基为WPM+ZT 2.0 mg·L-1,可获得较高的增殖倍率,且暗培养7 d可达最佳增殖效果;生根最适培养基为WPM+IBA 0.2 mg·L-1,生根率达86.67%。
Taking the healthy tender stem segments of wild Vaccinium dunalianum as explants, the media for axillary bud induction, subculture and rooting culture were selected. The results showed that the optimal medium for initial culture with an induction rate of 83.33% was WPM＋6-BA 3.0 mg·L^-1＋NAA 0.2 mg·L^-1, and the optimal medium for subculture was WPM＋ZT 2.0 mg·L^-1 with darkness cultured for 7 d. The optimal rooting medium was WPM＋IBA 0.2 mg·L^-1, with a rooting rate of 86.67%.
出处
《植物生理学报》
CAS
CSCD
北大核心
2014年第11期1717-1720,共4页
Plant Physiology Journal
基金
国家自然科学基金(21462040和31460076)
关键词
樟叶越桔
茎段
组织培养
快速繁殖
Vaccinium dunalianum
stem segment
tissue culture
rapid propagation
