摘要
为了缩短笃斯越桔的育苗周期,采用其腋芽进行组培技术育苗,该试验主要针对其初代培养技术进行研究,结果得出:采集笃斯越桔的腋芽,经过消毒后接种到MS+KT 0.6 mg/L培养基上,培养25d,挑选生长健壮的愈伤组织切成0.5cm小块,转入MS+6-BA 1.0 mg/L+NAA 0.2mg/L或MS+6-BA 1.0 mg/L+NAA 0.5 mg/L培养基上,可诱导分化出大量不定芽,然后利用这些不定芽作为中间繁殖体材料进行继代。
In order to shorten the days of nursery cycle for Vaccinium uliginosum, this experiment tryto use tissue culture technology to culture seedings by axillary bud, mainly aimed the induction stage.The results showed that the axillary buds of Vaccinium uliginosum were cut after disinfection, and wereinoculated into MS ＋ KT 0.6 mg/L medium for 25 days. The callus was grown and the callus was cutinto 0.5 cm pieces and transferred to MS ＋ 6-BA 1.0 Mg/L ＋ NAA 0.2 mg/L or MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.5 mg/L medium, could induce a large number of adventitious buds, and then could usethese adventitious buds for expanding.
作者
蔡柯
贾昊
姜茹桐
崔强
马贵义
建德锋
Cai Ke;Jia Hao;Jiang Rutong;Cui Qiang;Ma Guiyi;Jian Defeng(Jilin Agricultural Science and Technology University 132101)
出处
《现代农业研究》
2018年第5期9-10,122,共3页
Modern Agriculture Research
基金
吉林省大学生科技创新项目(吉农院合字[2018]第050号)
