摘要
通过ISSR分子标记技术分析18份来源于不同产地并经质量评价的草珊瑚种质的遗传多样性及亲缘关系,并进行亲本选配优化分析,为进一步利用这些种质进行育种奠定一定基础。应用ISSR-PCR分子标记技术研究18个份来自于不同居群的草珊瑚种质的DNA指纹图谱,应用Popgen32软件计算遗传多样性和遗传距离,并以UPCMA法进行聚类分析。经筛选获得23条多态性引物,ISSR-PCR扩增共获得198条谱带,其中多态性谱带184条,Nei’s基因多样性指数（h）为0.32,Shannon多样性指数（I）为0.485 4。供试的草珊瑚种质的遗传相似系数为0.383 8~0.878 8,平均为0.661 2。遗传距离分析揭示了各受试种质相互间的遗传一致性与遗传距离,其中种质S2与种质S18之间的遗传距离最远,为0.957 5,而种质S4与种质S5之间的遗传距离最近,仅为0.129 2。而聚类分析表明种质S1,S2,S3,S7,S10等5个种质在不同程度上有别于其余受试13个种质,并优化了S2与S3,S2与S6,S2与S18等3组较佳亲本选配组合。研究表明受试种质具有较好的遗传多样性,遗传距离分析与聚类分析所揭示的18个种质之间的亲缘关系,并为优化杂交育种的亲本选配提供遗传背景方面可靠的信息。
The study is aimed to assess the genetic diversity and genetic relationship of 18 Sarcandra glabra resources from different populations,and guide parent selection of cross breeding between these resources. The molecular marker technique ISSR was used to investigate the genetic diversity of the 18 resources. Data was analyzed by POPGEN 32, and a cluster diagram was presented by UPGMA. One hundred and ninety-eight amplified fragments were obtained using 23 ISSR primers. One hundred and eighty-four poly- morphic loci were identified. Nei＇s genetic diversity index （h） was 0.32, Shannon diversity index （I） was 0.485 4. The genetic similarity coefficient among the resources ranged from 0. 383 8 to 0. 878 8 in an average of 0. 661 2. The genetic distance between sample $2 and sample S18 was the farthest, so as between sample $3 and sample Sl8 both Nei＇s genetic distance was 0. 957 5, The genetic distance between sample S4 and sample S5 was the closest, the Nei＇s genetic distance was 0. 129 2, and the sample S1, S2, S3, S7, S10 were significantly different from the others based on the clustering analysis, the three groups S2 vs S3, S2 vs S6, S2 vs S18 were the best parent group selection. There was a middle level of genetic differentiation in the resources. The genetic distance between resources gives useful information to guide parent selection of cross breeding.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第23期4571-4575,共5页
China Journal of Chinese Materia Medica
基金
福建省教育厅A类项目(JA11138)
福建省科技重大项目(2011Y4005)
关键词
草珊瑚
ISSR
遗传多样性
亲缘关系
亲本选配
Sarcandra glabra
germplasm resource
genetic distance
cluster analysis
parent selection
