摘要
目的 观察肿瘤易感基因101（TSG101）对胃癌细胞血管形成的影响.方法 对不同TSG101表达水平的3组胃癌细胞亚系,分别进行体内实验和体外实验,检测血管内皮生长因子（VEGF）及其受体（VEGFR）的表达及血管形成.结果 （1）体外实验：TSG101组细胞促人脐静脉内皮细胞（HUVEC）成管数目明显多于空质粒组与未转染组[（19.50±3.02）个比（11.50±2.51）、（11.34±1.63）个,P＜0.05],TSG101组细胞VEGF分泌水平高于空质粒组与未转染组[（514.80±18.16） ng/L比（306.58±20.86）、（305.69±29.93） ng/L,P＜0.05];（2）体内实验：TSG101组荷瘤裸鼠瘤组织微血管密度和血清VEGF水平均明显高于空质粒组与未转染组[11.16±2.31比5.33±1.63、4.66±1.36,（340.64±19.15） ng/L比（219.34±13.56）、（206.10±25.85） ng/L,P＜0.05].TSG101组荷瘤裸鼠瘤组织中VEGF、VEGFR表达（0.548 ±0.032、0.345±0.042）明显高于未转染组（0.350±0.034、0.203±0.030）与空质粒组（0.327±0.020、0.206±0.036,P＜ 0.05）.结论 TSG101高表达的SGC7901细胞可能通过上调VEGF的表达促进胃癌血管形成.
Objective To investigate the effect of tumor susceptibility gene 101 （TSG101） on angiogenesis of gastric cancer SGC7901 cells.Methods Cells were divided into the non-transfection group,the empty plasmid transfection group and TSG101 eukaryotic expression plasmid transfection group （the TSG101 transfection group）.Then we examined the relationship between TSG101 expression and tumor angiogenesis in vivo and in vitro experiment respectively.To detect the expression of vascular endothelial growth factor （VEGF）,vascular endothelial growth factor receptor （VEGFR） and the angiogenesis.Resuits （1） It was showed that the angiogenesis number of HUVEC and the expression levels of VEGF in the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vitro experiment （19.50 ± 3.02 vs.11.50 ± 2.51,11.34 ± 1.63 and 514.80 ± 18.16 vs.306.58 ± 20.86,305.69 ± 29.93,all P 〈 0.05）.（2） It was showed the microvascular density in tumor tissue of nude mice and the serum VEGF concentration of the TSG101 transfection group was significantly higher than that of the empty plasmid transfection group and the non-transfection group in vivo experiment [11.16±2.31 vs.5.33 ±1.63,4.66 ±1.36 and （340.64±19.15） ng/L vs.（219.34 ± 13.56）,（206.10 ± 25.85） ng/L,all P 〈 0.05].The expressions of VEGF and VEGFR of the TSG101 transfection grou p （0.548 ± 0.032,0.345 ± 0.042） of tumor tissue in nude mice were significantly higher than that of the non-transfection group （0.350 ± 0.034,0.203 ± 0.030） and the empty plasmid transfection group （0.327 ± 0.020,0.206 ± 0.036,P 〈 0.05）.Conclusion The high-expression of TSG101 in SGC7901 cells may promote angiogenesis of gastric cancer by up-regulated expression of VEGF.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第11期2420-2422,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目（81071972）
关键词
肿瘤易感基因101
血管形成
血管内皮生长因子
胃癌
Tumor susceptibility gene 101
Angiogenesis
Vascular endothelial growth factor
Gastric cancer
