摘要
目的探讨不规范使用抗生素导致幽门螺杆菌(Hp)形成L型与感染复发的关系。方法以阿莫西林诱导Hp形成细胞壁缺陷型(即L型),以液体稀释法检测Hp及L型阿莫西林敏感性;以Hp灌胃感染小鼠,分别以生理盐水、常规或低于常规剂量阿莫西林或配伍克拉霉素治疗6d,20d后取胃组织行Hp及L型分离培养、16SrDNA片段PCR和病理学检查。结果阿莫西林对Hp和L型的MIC分别为0.0625和0.125μg/mL;单一阿莫西林治疗和感染对照小鼠胃粘膜L型分离、PCR均阳性,病理学检查呈慢性炎症表现;低剂量治疗和感染对照组均分离出Hp;配伍、盐水对照及空白组各项检测均阴性。结论不规范使用抗生素使Hp发生L型变异,L型生长或返祖可能是导致感染复发的重要原因。
Objective To explore the relationship between recurrence of Helicobacter pylori（Hp） infection and cell wall deficient forms of lip（L-forms of lip, HpL） induced by irregular antibiotic therapy. Methods L-forms of Hp were induced by amoxicillin. Amoxicillin sensitivity of Hp and HpL was detected by serial dilution test with liquid medium; Mice were infected by lavage with Hp, and then treated by normal saline, under-regular dose, regular dose of amoxicillin or accompaning with clarithromycin respectively everyday in 6 days. 20 days aiier final remedy, all mice were mercy killed and their gastric tissues were tested by PCR of Hp 16S rDNA ~agrnent, Hp and L-forms isolation and histopathologic examination. Results The minimal inhibition concentration of amoxicillin is 0.0625 and 0.125pg/mL on Hp and L-forms. All samples of infected mice untreated or treated by amoxicillin showed HpL isolation, PCR positive and chronic inflammation at different levels. Hp isolation of infected mice untreated or under-regular dose treated was positive. All test of combination treated group, the control and the blank group showed negative. Conclusion Cell wall deficient variance of Hp could be induced by irregular antibiotic therapy and lead to latent infection. The recurrence was possible because of slow growth or the atavistic variation of the L-form.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2013年第9期720-720,I0001-I0003,共4页
Chinese Journal of Antibiotics
基金
贵州省科技厅基金(黔科合J字(2008)2165号)
贵阳医学院博士基金(K2010-34)
关键词
幽门螺杆菌
细胞缺陷型
阿莫西林
Helicobacterpylori
Cell wall deficient forms（L-forms）
Amoxicillin
