摘要
目的观察骨髓基质细胞(BMSCs)经颈内动脉移植对短暂脑缺血大鼠的脑组织血管内皮生长因子(VEGF)表达及学习记忆的影响。方法 5只短暂脑缺血模型大鼠移植标记有18F-FDG的BMSCs,仅用于正电子发射型计算机断层显像(positron emission computed tomography,PET)观察。16只模型大鼠采用数字表法随机分为移植组(n=8)和对照组(n=8),另设假手术组(n=8)。移植组由颈内动脉种植BMSCs,对照组种植PBS,假手术组无特殊处理。14天后行脑组织HE染色,VEGF免疫组化染色。另设3个组,于移植后14和90天行Morris水迷宫实验。结果 BMSCs注射后3h,细胞主要分布于双侧颅内。移植后第14天,HE染色观察,与对照组比较,移植组神经元缺失较少,微血管增生明显。移植组在海马脑组织的VEGF阳性细胞数高于另两组,移植组为12.14±1.53个,对照组为9.53±1.31个,假手术组为7.23±0.77个,差异有统计学意义(P<0.05)。移植后第14天,移植组的定位航行潜伏期和穿越平台的次数为46.55±6.92s和4.50±1.05次,对照组为44.50±5.09s和5.50±1.05次,假手术组为27.83±5.80s和7.17±1.17次。移植组和对照组的定位航行潜伏期延长,穿越平台的次数减少,与假手术组比较,差异有统计学意义(P<0.05)。移植后第90天,移植组的定位航行潜伏期和穿越平台的次数为25.85±2.20s和7.50±1.05次,对照组为39.25±4.02s和5.83±0.75次,假手术组为24.62±2.63s和7.67±0.82次。对照组的定位航行潜伏期仍延长,穿越平台的次数仍减少,与另两组比较,差异有统计学意义(P<0.05)。结论 BMSCs移植可以减少短暂脑缺血大鼠神经细胞的丢失,促进脑组织VEGF的表达和血管增生,改善学习和记忆功能。
Objective To observe the effects of bone marrow stromal cell（BMSCs） transplantation on VEGF and learning and memory in rats with transient ischemia through internal carotid artery（ICA） injection.Methods Five model rats were transplanted with BMSCs labeled with 18F-FDG through ICA,then traced by Positron Emission Computed Tomography （PET）.Sixteen model rats were randomly divided into treated group （n =8） and control group （n =8）,sham-operated group （n =8） were also randomly selected.Treated group were injected with BMSCs through ICA,while control group with PBS,and sham-operated group without any treatment.After transplantation for 14d,the brain tissues were stained with hematoxylin-eosin （HE） stain and immunohistochemical stain of VEGF.Established three groups and were treated as same as the above of the second method.Behavioral tests were performed.Results PET scanning showed that the donor cells were primarily found in brains of the recipient rats 3 hours after transplantation.After transplantation for 14d,histologic analysis clearly demonstrated that the intra-arterially injected BMSCs significantly reduced neuronal loss and promoted microvascular proliferation.The number of VEGF positive cells in the hippocampus of the treated group rats were 12.14 ± 1.53,control group 9.53 ± 1.31,and sham-operated group 7.23 ±0.77.The number of VEGF positive cells in the treated group were higher than the other two groups （P 〈 0.05）.After transplantation for 14d,the escape latency and the number of passing the platform of the treated group were 46.55 ± 6.92s,4.50 ± 1.05times,control group 44.50 ± 5.09s,5.50 ± 1.05times and sham-operated group 27.83 ± 5.80s,7.17 ± 1.17 times.The results clearly showed that the escape latency was longer and the number of passing the platform were less in the treated group and control group than the sham-operated group （P 〈 0.05）.After transplantation for 90d,the escape latency and the number of passing the platform in the treated group were 25.8
出处
《医学研究杂志》
2014年第10期110-114,共5页
Journal of Medical Research
