摘要
目的观察颈内动脉移植骨髓间充质干细胞(BMSCs)透过血脑屏障(BBB)在血管性痴呆(VD)大鼠脑内存活、迁移及对认知能力的影响。方法分离大鼠股骨骨髓单核细胞,以贴壁培养法培养BMSCs,分化诱导并作BrdU标记;选72只Wistar大鼠分为对照组、模型组、治疗组3组,制作VD大鼠模型,治疗组于术后24h颈内动脉移植0.5mL 1.2×107/mL的BrdU标记细胞。移植4、8周后检测移植细胞在VD大鼠脑内存活、迁移以及大鼠学习记忆能力。结果颈内动脉移植后骨髓间充质干细胞广泛存活于VD大鼠脑组织内,随着时间的延长,迁移方向集中,主要聚集至海马、大脑皮质等缺血易损伤区域。移植后4、8周,模型组大鼠主动回避反应(AAR)比率[(42.1±4.5)、(43.6±3.6)]低于对照组[(90.0±4.3)、(92.5±5.0)],差异有统计学意义(P<0.01);治疗组(69.2±4.7,70.8±4.7)较模型组明显提高,差异有统计学意义(P<0.01)。结论骨髓间充质干细胞颈内动脉移植后可透过BBB向脑缺血易损伤部位迁移聚集、存活,促进缺颈内动脉移植后骨血损伤后脑组织的修复与再生,明显增强VD大鼠学习记忆能力。
Objective To observe the survival and migration characteristics after intracarotid transplantation of bone marrow stem cells（BMSCs） and its effect on learning-memory abilities across the blood-brain barrier（BBB） in the vascular dementia（VD） rats .Methods Bone mononuclear cells（BMNCs） were isolated from bone marrow in vitro by standard Ficoll-Hypaque technique , then cells were enriched and expanded by using bone marrow adherent culture .72 Wistar rats were meanly divided into control group ,model group and treatment group .The VD rat model was established by modified pulsinellis 4-vessel occlusion（4 VO） .The treatment group received intracarotid infusion of 0 .5 mL 1 .2 × 107/mL BMSCs which were labeled with BrdU in vitro after opera-tion .Their survival ,migration and the learning-memory abilities were observed at 4th and 8th week .Results BMSCs transplanted by intracarotid transplantation survived and had been found throughout the brain tissue .They major migrated and localized in the is-chemic zone of injury such as hippocampus and cerebral cortex .Compared with the control group ,active avoidance response（AAR） ratio in the model group（42 .1 ± 4 .5） ,group（43 .6 ± 3 .6）showed significantly decrease compared with the control group （90 .0 ± 4 .3） ,（92 .5 ± 5 .0）（P〈0 .01） ,and the treatment group（69 .2 ± 4 .7） ,（70 .8 ± 4 .7）was significant higher than the model group（P〈0 .01） .Conclusion Intracarotid transplantation of BMSCs could enter the VD rats cerebra parenchyma via BBB ,migrate into the damaged brain tissue to gather and survive .Learning-memory abilities can be improved significantly by transplanted BMSCs .
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第36期4364-4367,共4页
Chongqing medicine
基金
军队"十一五"科技攻关项目(06G076)
关键词
间质干细胞移植
骨髓颈内动脉
痴呆
血管性
细胞运动
学习记忆
mesenchymal stem cell trans plation
bone marrow intracarotid
dementia, vascular
cell movement
learning-memory
