摘要
目的探讨慢性乙型肝炎病毒(HBV)感染患者血清乙型肝炎病毒外膜大蛋白(HBV-LP)与HBV-DNA、HBeAg之间的关系及临床应用价值。方法 2010年10月-2011年5月采用酶联免疫吸附法(ELISA)检测乙型肝炎病毒患者血清中HBV-LP,化学发光免疫分析法(CLIA)检测乙型肝炎病毒标志物(HBV-M),实时荧光定量PCR法(RT-PCR)检测HBV DNA载量,比较分析三者间的阳性率及相关性,数据采用SPSS17.0软件进行统计分析。结果 1 036例慢性HBV感染者中,HBV-LP阳性率为84.36%,较HBV-DNA阳性率72.01%与HBeAg阳性率54.44%均高,差异有统计学意义(P<0.01);HBV-LP与HBV-DNA和HBeAg的总符合率分别为78.19%、64.48%,HBV-LP与HBV-DNA和HBeAg阳性符合率分别为93.43%、94.86%;HBV-LP表达与HBV-DNA和HBeAg之间均显著关联(χ2=101.67,χ2=65.35),差异有统计学意义(P<0.01)。结论 HBVLP是反映HBV感染者体内复制程度的良好血清免疫学指标,血清中HBV-LP与HBV-DNA具有较好的相关性,特别是可作为HBeAg阴性患者检测病毒复制及预后判断的良好的指标。
OBJECTIVE To discuss the relationship between HBV-LP , HBV-DNA and HBeAg in patients with HBV infection in Lanzhou ,and its clinical application .METHODS ELISA ,CLIA and RT-PCR were used to detect HBV-LP ,HBV-M and HBV DNA ,respectively ,and the relativity between them was analyzed .The data were analyzed by SPSS17 .0 software .RESULTS In 1036 patients ,the positive rate of HBV-LP was 84 .36% ,higher than HBV DNA （72 .01% ） and HBeAg （54 .44% ） ,the difference was significant（P〈0 .01） .The total consistent rates of HBV-LP to HBV-DNA and HBeAg were 78 .19% ,64 .48% .The positive consistent rates of HBV-LP to HBV-DNA and HBeAg were 93 .43% ,94 .86% . HBV-LP expression with HBV-DNA and HBeAg showed significant correlation （χ2 = 101 .67 ,χ2 = 65 .35） ,and the difference was significant （P〈0 .01） .CONCLUSION HBV-LP is a good serum immune index of HBV replication in HBV infected patients , HBV-LP shows good relativity with level of HBV-DNA ,especially when using as an index of HBV replication and prognosis judgment in HBeAg negative serum patients .
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2014年第20期4976-4978,共3页
Chinese Journal of Nosocomiology
基金
兰州市科技局基金资助项目(2011-ZD-08)
