摘要
目的:了解HBV外膜大蛋白(HBV-LP)对HBV感染、复制和乙肝患者诊断、治疗和预后评估的作用。方法:收集104例乙型肝炎患者血清(HBsAg阳性),同时检测血清HBV-M、HBV-LP、HBV DNA,HBV-LP检测采用ELISA法,HBV DNA由广州达安基因股份有限公司提供检测;测定采用FQ PCR。结果:不同HBV-M模式中,HBV DNA、HBV-LP阳性检出率差异无统计学意义(P>0.05),HBV DNA含量(拷贝数对数)与HBV-LP浓度均值呈正相关关系。在72例血清HBV DNA阳性患者中,HBV-LP阳性65例,阳性率90.3%。结论:HBV-LP在不同HBV-M感染模式中,与HBV DNA的阳性率比较吻合,而且HBV-LP浓度能较好的反映体内HBV DNA的存在状态。
Objective：To investigate the role of HBV large surface protein （HBV-LP） in HBV infection, replication, as well as diagnosis, treatment and prognosis of HBV patients. Methods. Collecting the serum samples of 104 HBV patients （HBsAg positive）, detecting serum HBV-M, HBV-LP and HBV DNA. HBV-LP was detected by ELISA. HBV DNA, which was measured by FQ PCR, was detected by Da An Gene Co, Ltd. of Guangzhou province. Results. In different HBV-M modes, the difference of positive rate between HBV DNA and HBV-LP was not statistically significant （P〉0. 05）. HBV DNA content （logarithm of the copy number） was positively correlated with the mean concentration of HBV-LP. For 72 cases of serum HBV DNA-positive patients, 65 cases were HBV-LP positive, the positive rate was 90. 3%. Conclusion. For different HBV-M infection patterns, HBV-LP is consistent with the positive rate of PIBV DNA. And HBV-LP concentrations can suitably reflect the state of HBV DNA presence in vivo.
出处
《医学理论与实践》
2013年第9期1138-1139,1159,共3页
The Journal of Medical Theory and Practice
