摘要
目的分析慢性乙肝(chronic hepatitis B,CHB)患者乙型肝炎病毒外膜大蛋白(HBV-LP)、前S1抗原(Pre S1-Ag)与病毒复制状态的相关性。方法采用回顾性研究,收集78例CHB患者血清标本,应用实时荧光定量聚合酶链反应测定血清乙型肝炎病毒DNA(HBV-DNA),根据样品定量水平计算阳性率;应用酶联免疫吸附试验法测定Pre S1-Ag、乙肝表面抗原(HBsAg)、乙肝核心抗体(HBcAb)、乙肝e抗原(HBeAg)、乙肝e抗体(HBeAb)和HBV-LP阳性情况;分析HBV-DNA与HBV-LP、Pre S1-Ag的相关性。结果78例CHB患者Pre S1-Ag、HBV-LP和HBV-DNA阳性率分别为34.62%、70.51%、65.38%,差异有统计学意义(χ2=23.97,P<0.05)。比较CHB患者测定的二对半指标不同组合,HBeAg阴、阳性患者的Pre S1-Ag、HBV-LP和HBV-DNA阳性率差异均有统计学意义(P值均<0.05);HBcAb+HBsAg阳性、HBcAb+HBeAb+HBsAg阳性、HBcAb+HBeAg+HBsAg阳性患者的Pre S1-Ag和HBV-DNA阳性率比较,差异均有统计学意义(P值均<0.05);HBeAg+HBsAg阳性、HBeAb+HBsAg阳性患者的Pre S1-Ag、HBV-LP和HBV-DNA阳性率比较,差异均无统计学意义(P值均>0.05)。随着HBV-DNA载量的升高,HBV-LP和Pre S1-Ag吸光度值亦随之升高(P值均<0.05)。相关性分析显示,HBV-DNA载量与HBV-LP吸光度值呈现正相关关系(r=0.83,P<0.05),与Pre S1-Ag吸光度值呈现正相关关系(r=0.65,P<0.05)。结论CHB患者血清HBV-DNA、HBV-LP和Pre S1-Ag阳性率比较,存在显著差异,并且患者血清HBV-DNA载量与HBV-LP存在显著相关性,HBV-LP可作为HBV标志物的重要补充,用于评价患者病毒复制状态。
Objective To analyze the serum hepatitis B virus large surface protein(HBV-LP)and pre S1 antigen(Pre S1-Ag)in patients with chronic hepatitis B(CHB)and their correlation with viral replication status.Methods The retrospective study was conducted.The serum samples of 78 CHB patients were collected and subjected to real-time fluorescent quantitative PCR analysis for HBV-DNA.The positive rate was calculated according to the quantitative results of samples;the positive rates of PreS1 Ag,HBsAg,HBcAb,HBeAg,HBeAb and HBV-LP were determined by enzyme-linked immunosorbent assay;the correlations between HBV-DNA and HBV-LP,Pre S1-Ag were analyzed.Results The positive rates of Pre S1-Ag,HBV-LP and HBV-DNA in 78 patients were 34.62%,70.51%and 65.38%,respectively,with significantly difference(P<0.05).The combinations of indexes were compared:the positive rates of Pre S1-Ag,HBV-LP and HBV-DNA in HBeAg negative and positive patients were significantly different(all P<0.05);there were significant differences in Pre S1-Ag,HBV-LP and HBV-DNA positive rates among the HBcAb+HBsAg,HBcAb+HBeAb+HBsAg and HBcAb+HBeAg+HBsAg positive patients(all P<0.05).In HBeAg+HBsAg positive and HBeAb+HBsAg positive patients,there were no significant differences in the positive rates of Pre S1-Ag,HBV-LP and HBV-DNA(all P>0.05).With the increase of HBV-DNA load,the absorbance values of HBV-LP and Pre S1-Ag also increased(P<0.05).Pearson correlation analysis showed that HBV-DNA load was positively correlated with both absorbance value of HBV-LP(r=0.83,P<0.05)and Pre S1-Ag(r=0.65,P<0.05).Conclusion The positive rates of HBV-DNA,HBV-LP and Pre S1-Ag in CHB patients were significantly different,and there was a significant correlation between HBV-DNA load and HBV-LP.HBV-LP can be used as an important supplement of HBV markers to evaluate the viral replication status of patients.
作者
王世全
朱康宁
张琦
WANG Shi-quan;ZHU Kang-ning;ZHANG Qi(The First Outpatient Department of Henan Provincial Organs,Henan Zhengzhou,45000,China;不详)
出处
《江苏预防医学》
CAS
2021年第2期157-159,166,共4页
Jiangsu Journal of Preventive Medicine
