摘要
目的：探讨结缔组织生长因子（CTGF）蛋白、转化生长因子-β1（TGF-β1）蛋白在宫颈鳞癌组织中的表达及其与临床病理特征的关系,特别是与盆腔淋巴结转移的关系.方法：应用Power Vision免疫组织化学染色法检测正常宫颈组织（10例）、CINⅡ～Ⅲ组织（20例）和宫颈鳞癌组织（98例）中CTGF蛋白及TGF-β1蛋白的表达情况,并比较两种蛋白的阳性表达率与宫颈鳞癌的不同临床病理因素间的关系及其与盆腔淋巴结转移的关系.结果：①CTGF蛋白、TGF-β1蛋白在正常宫颈、CINⅡ～Ⅲ、宫颈鳞癌中的阳性表达率呈现递增的趋势,三者比较差异有统计学意义（P＜0.05）.②宫颈鳞癌中CTGF蛋白表达与TGF-β1蛋白表达之间呈正相关（r=0.643,P＜0.05）.③宫颈鳞癌组织中CTGF、TGF-β1蛋白的阳性表达率在不同的组织学分化程度、盆腔淋巴结有无转移和不同临床分期间比较,差异有统计学意义（P＜0.05）.④多因素非条件Logistic回归分析示：CTGF蛋白、年龄、组织分化程度及肿瘤直径是影响宫颈鳞癌盆腔淋巴结转移的独立危险因素（P＜0.05）.结论：CTGF蛋白、TGF-β1蛋白参与宫颈鳞癌的恶性程度进展,且CTGF可作为宫颈鳞癌盆腔淋巴结转移的独立危险因素之一.
Objective： To investigate the expression and significance of connective tissue growth factor （CTGF） protein and transforming growth factor-β1 （TGF-β1）protein in cervical squamous cell carcinoma tis- sue, especially the its correlation with pelvic lymphatic metastasis. Methods ：The expression of connective tis- sue gt＇owth factor（ CTGF） protein and transforming growth factor-β1 （TGF-β1）protein in 10 cases of normal cervical tissue,20 cases of CIN Ⅱ-Ⅲ and 98 cases of cervical squamous cell carcinoma tissues were detec- ted by immunohistochemistry SP method. The expression levels of these two proteins in cervical squamous tissue and its correlations with pelvic lymphatic metastasis were analyzed. Results ：①The positive expression rates of CTGF and TGF-β1 in the normal cervix,CIN Ⅱ -Ⅲ ,cervical squamous cell carcinoma tissue appeared increasing trend, the difference among these three groups was statistically significant（ P 〈 0.05）. ②CTGF and TGF-β1 expressions were positively correlated in cervical squamous cell carcinoma（r=0. 643,P〈0. 05）,③The expressions of CTGF and TGF-β1 protein in cervical squamous cell carcinoma were related to differ- entiation of cervical squamous cell carcinoma, lymph-node involvement and clinical stages （ P 〈 0. 05）. ④Mul- tivariate Logistic regression analysis showed that CTGF protein, age, histological grade and tumor diameter were the independent risk factors of pelvic lymphatic metastasis of cervical squamous cell carcinoma. Con- cluslons.CTGF and TGF-β1 are involved in the development and invasion of cervical squamous cell carcino- ma. High level CTGF maybe an independent risk factor of pelvic lymphatic metastasis of cervical squamous cell carcinoma.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2014年第8期600-604,共5页
Journal of Practical Obstetrics and Gynecology
基金
湖南省科学技术厅基金资助(编号:2012FJ4068)
关键词
宫颈鳞癌
结缔组织生长因子
转化生长因子-Β1
免疫组织化学法
Cervical squamous cell carcinoma
Connective tissue growth factor
Transforming growth factor-β1
Immunohistochemistry
