摘要
目的 观察血管紧张素Ⅱ（AngⅡ）、卡托普利对体外培养的肝星状细胞金属蛋白酶组织抑制因子-1（TIMP-1）基因表达的影响.方法 采用肝星状细胞株HSC-T6作为活化的肝星状细胞的研究模型,将培养的肝星状细胞随机分为对照组、AngⅡ（1×10-5、1 ×10-7 mol/L）组和AngⅡ±卡托普利组（1×10-5、1×10-7 mol/L）.逆转录-聚合酶链反应（RT-PCR）法检测肝星状细胞中TIMP-1mRNA的表达.结果 TIMP-1基因表达水平在AngⅡ两组分别是1.145 ±0.219、0.860±0.115,而对照组为0.523±0.056,TIMP-1基因的表达水平在不同浓度的AngⅡ＋卡托普利组（1×10-5 mol/L、1×10-7 mol/L）分别是0.740±0.089、0.590±0.073.结论 AngⅡ能够促进肝星状细胞TIMP-1 mRNA的表达,而卡托普利能够明显抑制这一作用.
Objective To investigate the effects of angiotensin Ⅱ （Ang Ⅱ） and captlpril on tissue inhibitorof metalloproteinase-1 （TIMP-1） mRNA expression in culured hepatic stellate cells （HSCs） in vivo.Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated HSCs.The cultured HSCs were randomized into control group,Ang Ⅱ group （1 × 10-5,1 × 10-7 mol/L） and AngⅡ ＋ captlpril group （1 × 10-5,1 × 10-7 mol/L）.HSCs were harvested to measure the TIMP-1 mRNA expression by reverse transcription-polymerase chain reaction （RT-PCR）.Results TIMP-1 gene expression levels in different groups of Ang Ⅱ （1 × 10-5 mol/L,1 × 10-7 mol/L） were 1.145 ±0.219 and 0.860±0.115 respectively,and those in control group was 0.523 ± 0.056.TIMP-1 expression levels in different groups of Ang Ⅱ ± captlpril （1 × 10-5 mol/L,1 × 10-7 mol/L） were 0.740 ± 0.089 and 0.590 ± 0.073 respectively,and those in Ang Ⅱ group were 0.850 ± 0.107 and 0.620 ± 0.103 respectively.Conclusion Angiotensin Ⅱ can increase the type Ⅰ collage mRNA expression of HSCs,which can be inhibited by captlpril.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2014年第8期1729-1730,共2页
Chinese Journal of Experimental Surgery
