摘要
目的观察重组人肝再生增强因子（hALR）对大鼠肝星状细胞c—fos基因表达的影响。方法在培养的肝星状细胞株中加入高（200μg／L）、中（20μg／L）、低（2μg／L）3种浓度的hALR，于8、24、48、72h四个时间点收集细胞，提取总RNA；用逆转录定量聚合酶链反应（PCR）方法测定c—fos的基因表达。结果高、中、低浓度的hALR3组肝星状细胞c-fos基因的表达水平在8、24、48、72h四个时间点均明显低于对照组；低剂量组为对照组的50％～53％，中剂量组为对照组的38％-43％，高剂量组为对照组的26％～30％。结论重组人肝再生增强因子对大鼠肝星状细胞c—fos基因的表达有明显抑制作用。
Objective To investigate the effects of recombinant human augmenter of liver regeneration （hALR） on c-fos gene expression in hepatic stellate ceils （HSCs）. Methods HSC-T6 cells were cultured in medium containing different concentrations of hALR （200,20,2 μg/L respectively）, and collected at different incubation periods （ 8,24,48,72 h）. Total RNA of HSCs was isolated, and the c-fos gene expressions levels were detected by reverse transcription polymerase chain reaction. Results The C- fos gene expression levels of HSCs in high （200 μg/L）,middle （20 μg/L） and low （2 g/L） concentrations of hALR groups were significantly lower than those in control group after exposure to hALR for 8,24, 48 and 72 h. The expression of C-fos gene in low,middle,and high hALR groups was 50%-53% ,38%- 43% ,and 26%-30% of control group,respectively. Conclusion hALR could inhibit the gene expression of c-fos in HSCs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第7期900-901,共2页
Chinese Journal of Experimental Surgery
基金
军队“十五”杰出人才基金资助项目（01J002）
军队“十一五”面上A类基金（06MA073）资助项目
