摘要
目的观察丹参对肝星状细胞c—fos和c-jun基因表达的影响。方法在培养的肝星状细胞株中分别加入不同浓度的丹参（终质量浓度分别为0．8、0．4g／L）；于8、24、48、72h4个时间点分别收集细胞，提取肝星状细胞总RNA；用逆转录定量聚合酶链反应（RT—qPCR）方法测定c—fos和c-jun的基因表达水平。结果丹参两组HSC—T细胞c—fos基因表达水平在8h（0．30±0．08、0．48±0．11）、24h（0．26±0．09、0．43±0．10）、48h（0．20±0．06、0．36±0．09）、72h（0．13±0．04、0．23±0．06）4个时间点均明显低于对照组（0．63±0．13、0．50±0．12、0．43±0．06、0．32±0．04）；c-jun基因表达水平在8h（0．43±0．12、0．69-t-O．14）、24h（0．38±0．09、0．56±0．10）、48h（0．27±0．08、0．46-4-0．10）、72h（0．20±0．04、0．34±0．06）4个时间点也均明显低于对照组（0．93±0．13、0．80±0．12、0．63±0．10、0．46±0．07）；随剂量加大，HSC-T细胞c—fos、c-jun基因表达水平逐渐降低；两组问差异有统计学意义（P〈0．01）。结论丹参对肝星状细胞c—fos和c-jun基因表达有明显的下调作用。
Objective To investigate the effects of salvia miltiorrhiza on c-fos and c-jun gene ex- pression in hepatic stellate cells （HSCs）. Methods HSCs were cultured in medium containing different concentrations of salvia miltiorrhiza （0. 8 and 0.4 g/L） and harvested at different incubation periods （8, 24, 48 and 72 h）. Total RNA of HSCs was isolated, and the expression levels of c-fos and c-jun genes were detected by using reverse transcription polymerase chain reaction. Results C-fos gene expression lev- els of HSC in two salvia miltiorrhiza-treated groups were significantly lower than those in control group （0.63 ±0. 13, 0.50 ±0. 12, 0.43 ±0.06, 0.32 ±0.04） after exposure to salvia mihiorrhiza at 8 h （0. 30 ＋ 0. 08, 0. 48 ± 0. 11 ）, 24 h （0. 26 ＋ 0.09, 0. 43 ± 0. 10）, 48 h （0. 20 ± 0. 06, 0. 36 ± 0. 09） and 72 h （0. 13 ±0. 04, 0. 23 ±0. 06）. C-jun gene expression levels of HSCs in two salvia miltiorrhiza-treated groups were also significantly lower than those in control group （0. 93 ± 0. 13, 0. 80 ± 0. 12, 0. 63 ± 0. 10, 0. 46 ± 0. 07 ） after exposure to salvia miltiorrhiza at 8 h （ 0.43 ± 0. 12, 0. 69 ± 0. 14 ）, 24 h （ 0. 38 ± 0.09, 0.56±0.10）,48h （0.27±0.08, 0.46±0.10） and 72h （0.20±0.04, 0.34±0.06）. The c-fos and c-jun gene expression levels of HSCs were decreased gradually as the dosage of salvia mihiorrhiza increased and there were significant differences in c-fos andc-jun gene expression between two salvia mihi- orrhiza-treated groups. Conclusion Salvia miltiorrhiza may down-regulate c-fos and c-jun gene expression in HSCs.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第2期288-289,共2页
Chinese Journal of Experimental Surgery
基金
军队“十五”杰出人才基金课题（01J002）
军队“十一五”面上课题（06MA073）
