摘要
以野生香椿种子为外植体进行培养,将香椿种子用75%酒精溶液消毒20s,再用0.1%升汞溶液消毒5min,无菌水冲洗4次~6次,接种到培养基上。在接种8d^10d后外植体分化出带有一片小叶的新生枝。切取子叶和下胚轴作为外植体接种B5+蔗糖30g/L的培养基中,10d后形成增殖苗。通过组织培养技术,缩短香椿的生长周期,再将香椿的嫩芽做如下处理浸泡(料液比1∶4,0.05%碳酸钠)、清洗、浸烫(95℃水浴2min,500mg/kg的葡萄糖酸锌溶液)、沥干冷却、切碎、拌料、加入添加剂、装罐、杀菌(85℃,30min)、冷却、贮藏。
This topic is based on the seeds of wild toon were cultured as explants, the toon seeds with 75%alcohol solution to disinfect 20 s, and then 0.1%mercuric chloride solution In disinfection 5min, the sterile water 4 to 6 times, inoculated into the medium. Explants differentiation after inoculated 8 d-10 d, with a lobular new branches. Cut to take cotyledon and hypocotyl as explants inoculated B5＋sucrose 30 g/L medium, after 10 days form the proliferation of seedlings. By tissue culture techniques to shorten the growth cycle of the toon , and then toon buds do the following processing immersion （1∶4,0.05%sodium carbonate）-Cleaning-scalding liquid ratio （95℃water bath for 2 min, 500 mg/kgglucose solution of zinc）-drain the cooling-chopped-spices-mix in additives-canning-sterilization （85 ＆#176; C, 30 min）-cooling-storage.
出处
《食品研究与开发》
CAS
北大核心
2014年第13期21-23,共3页
Food Research and Development
关键词
香椿
组织培养
工艺技术
tissue culture
cedrela sinensis
technology
