摘要
本文以尚未完全成熟的香椿种子为研究对象,首次建立了基于种皮愈伤组织诱导与分化的香椿再生技术体系。消毒后的种子在MS+0.01 mg·L^(-1)TDZ+0.3 mg·L^(-1)NAA+0.01 mg·L^(-1)2,4-D+3%蔗糖的培养基诱导种皮愈伤组织,诱导率100%。在3/4MS+1.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)IAA+3%蔗糖培养基进行愈伤组织分化,不定芽分化率53.33%,1块愈伤组织一般可形成3~5个丛芽。不定芽生长4个月后,将无根试管苗基部用200 mg·L^(-1)IBA浸泡处理2 min后,在1/2MS+3%蔗糖下培养,生根率达85.60%。本研究为香椿遗传转化及功能基因研究和优质苗木的无性快速繁殖奠定了基础。
The immature Toona sinensis seeds were used as the research object,a regeneration technology system based on seed coat callus induction and differentiation was established in T.sinensis.The sterilized seeds were treated with MS+0.01 mg·L^(-1)TDZ+0.3 mg·L^(-1)NAA+0.01 mg·L^(-1)2,4-D+3%sucrose to induce seed coat callus,and the induction rate was 100%.The differentiation rate of adventitious buds was 53.33%in the media of 3/4MS+1.5 mg·L^(-1)6-BA+0.1 mg·L^(-1)IAA+3%sucrose.In general,3 to 5 clump buds could be formed from one callus.After 4 months of adventitious bud growth,the rooting rate was 85.60%in the media of 1/2MS+3%sucrose after soaking the base of rootless tube seeding with 200 mg·L^(-1)IBA for 2 minutes.This study laid a foundation for the genetic transformation and functional gene research of T.sinensis and the asexual and rapid reproduction of high-quality seedlings.
作者
李玲
黄榕
艾薇
王友如
LI Ling;HUANG Rong;AI Wei;WANG Youru(College of Life Sciences,Hubei Normal University,Huangshi,Hubei 435002,China;Hubei Engineering Research Center for Breeding and Comprehensive Utilization of Characteristic Wild Vegetable,Huangshi,Hubei 435002,China;Hubei Key Laboratory of Conservation and Utilization of Edible Wild Plants,Huangshi,Hubei 435002,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2023年第3期599-605,共7页
Plant Physiology Journal
基金
湖北省技术创新专项(重大项目)(2019ABA112)。
关键词
香椿
种皮
组织培养
再生技术
Toona sinensis
seed coat
tissue culture
regeneration technology
